EC: 6.1.1.24

glutamate-tRNAGln ligase (L-glutamate:tRNAGlx ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P22250 [Regulatory factor beta]

L_Glutamate + ATP + tRNA(Gln) -> AMP + L_Glutamyl_tRNA(Gln) + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Sodium HEPES, 10 mM KCl, 10 mM MgCl2
PH: 7.2
Temperature: 30
uniprot:P22250 [Regulatory factor beta]

ATP + L_Glutamate + tRNA(Gln) -> Diphosphate + L_Glutamyl_tRNA(Gln) + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Sodium HEPES, 10 mM KCl, 10 mM MgCl2
PH: 7.2
Temperature: 30
uniprot:P22250 [glutamate---tRNAGln ligase(Enzyme) wildtype]

ATP + tRNA(Gln) + L_Glutamate -> AMP + Diphosphate + L_Glutamyl_tRNA(Gln)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Sodium HEPES, 10 mM KCl, 10 mM MgCl2
PH: 7.2
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-HSA-72766 Translation
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency