EC: 6.1.1.19

arginine-tRNA ligase (L-arginine:tRNAArg ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P11875 [N(omega)-(L-Arginino)succinate]

L_Arginine + tRNA(Arg) + ATP -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.4
Temperature: 37
uniprot:P11875 [arginine---tRNA ligase(Enzyme) wildtype]

tRNA(Arg) + ATP + L_Arginine -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.4
Temperature: 37
uniprot:P11875 [N(omega)-(L-Arginino)succinate]

tRNA(Arg) + L_Arginine + ATP -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.4
Temperature: 37
uniprot:P11875 [N(omega)-(L-Arginino)succinate]

tRNA(Arg) + L_Arginine + ATP -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Hepes, 10 mM MgCl2, 1 mM dithioerythritol, 0.5 mM ornithine, 0.5 mM citrulline
PH: 7.4
Temperature: 37
uniprot:P11875 [N(omega)-(L-Arginino)succinate]

ATP + tRNA(Arg) + L_Arginine -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Hepes, 10 mM MgCl2, 1 mM dithioerythritol, 0.5 mM ornithine, 0.5 mM citrulline
PH: 7.4
Temperature: 37
uniprot:P11875 [arginine---tRNA ligase(Enzyme) wildtype]

tRNA(Arg) + L_Arginine + ATP -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (k * C0) ) - (( (k + k_b) ) * P)) buffer: 0.1 M Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.4
Temperature: 37
uniprot:P11875 [arginine---tRNA ligase(Enzyme) wildtype]

tRNA(Arg) + L_Arginine + ATP -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.4
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

tRNA(Arg) + L_Arginine + ATP -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

ATP + tRNA(Arg) + L_Arginine -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

L_Arginine + ATP + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

ATP + tRNA(Arg) + L_Arginine -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

tRNA(Arg) + ATP + L_Arginine -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

ATP + tRNA(Arg) + L_Arginine -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

ATP + L_Arginine + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

L_Arginine + tRNA(Arg) + ATP -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

tRNA(Arg) + L_Arginine + ATP -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

tRNA(Arg) + ATP + L_Arginine -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

tRNA(Arg) + ATP + L_Arginine -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

ATP + tRNA(Arg) + L_Arginine -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

L_Arginine + ATP + tRNA(Arg) -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

tRNA(Arg) + L_Arginine + ATP -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 10 mM MgCl2, 0.1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

L_Arginine + ATP + tRNA(Arg) -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

ATP + L_Arginine + tRNA(Arg) -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

tRNA(Arg) + ATP + L_Arginine -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

ATP + tRNA(Arg) + L_Arginine -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

ATP + L_Arginine + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

tRNA(Arg) + ATP + L_Arginine -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

tRNA(Arg) + ATP + L_Arginine -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

L_Arginine + tRNA(Arg) + ATP -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

L_Arginine + ATP + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

ATP + L_Arginine + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

ATP + tRNA(Arg) + L_Arginine -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

tRNA(Arg) + L_Arginine + ATP -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

ATP + L_Arginine + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

tRNA(Arg) + L_Arginine + ATP -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

L_Arginine + tRNA(Arg) + ATP -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

tRNA(Arg) + ATP + L_Arginine -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

tRNA(Arg) + L_Arginine + ATP -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

ATP + tRNA(Arg) + L_Arginine -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

L_Arginine + tRNA(Arg) + ATP -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

ATP + tRNA(Arg) + L_Arginine -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

ATP + L_Arginine + tRNA(Arg) -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

ATP + L_Arginine + tRNA(Arg) -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

ATP + L_Arginine + tRNA(Arg) -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

ATP + L_Arginine + tRNA(Arg) -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

tRNA(Arg) + L_Arginine + ATP -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

L_Arginine + tRNA(Arg) + ATP -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

ATP + L_Arginine + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

tRNA(Arg) + ATP + L_Arginine -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRSdeltaN]

tRNA(Arg) + L_Arginine + ATP -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

ATP + L_Arginine + tRNA(Arg) -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y]

tRNA(Arg) + L_Arginine + ATP -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

L_Arginine + ATP + tRNA(Arg) -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/M222I]

L_Arginine + ATP + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

ATP + L_Arginine + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

tRNA(Arg) + L_Arginine + ATP -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

L_Arginine + ATP + tRNA(Arg) -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

L_Arginine + tRNA(Arg) + ATP -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant M222I]

L_Arginine + ATP + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

L_Arginine + ATP + tRNA(Arg) -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant C599Y/Y506S]

tRNA(Arg) + L_Arginine + ATP -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

tRNA(Arg) + ATP + L_Arginine -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 37
uniprot:P37880 [arginine---tRNA ligase(Enzyme) mutant Y506S]

L_Arginine + ATP + tRNA(Arg) -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (E * (( (( (kcat_1 * A) ) / Km_1) ) + (( (( (kcat_2 * (A ^ 2)) ) / Km_1) ) * Km_2))) ) / (( (1 + (A / Km_1)) ) + (( (( (A ^ 2) ) / Km_1) ) * Km_2))) buffer: 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl, 0.5 mM dithioerythritol
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

tRNA(Arg) + L_Arginine + ATP -> L_Arginyl_tRNA(Arg) + Diphosphate + AMP		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 0.5 mM DDT, 5 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS-deltaN]

ATP + tRNA(Arg) + L_Arginine -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 0.5 mM DDT, 5 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS]

tRNA(Arg) + L_Arginine + ATP -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 0.1 mM EDTA, 10 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS-deltaN]

L_Arginine + ATP + tRNA(Arg) -> AMP + Diphosphate + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 0.1 mM EDTA, 10 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS-Cx]

L_Arginine + ATP + tRNA(Arg) -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 0.1 mM EDTA, 10 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS-Cx/Casp7]

ATP + tRNA(Arg) + L_Arginine -> Diphosphate + AMP + L_Arginyl_tRNA(Arg)		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 0.1 mM EDTA, 10 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS-Cx/Casp7]

L_Arginine + ATP + tRNA(Arg) -> Diphosphate + L_Arginyl_tRNA(Arg) + AMP		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 0.5 mM DDT, 5 mM MgCl2
PH: 7.5
Temperature: 25
uniprot:P37880 [arginine---tRNA ligase(Enzyme) wildtype ArgRS-Cx]

L_Arginine + ATP + tRNA(Arg) -> AMP + L_Arginyl_tRNA(Arg) + Diphosphate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 20 mM imidazole-HCl, 150 mM KCl, 0.5 mM DDT, 5 mM MgCl2
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:CAULONA1000_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:PDIF272563_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL199310_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
Reactome:R-BTA-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-RNO-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-BTA-9730414 MITF-M-regulated melanocyte development
Reactome:R-DME-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-HSA-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9730414 MITF-M-regulated melanocyte development
Reactome:R-RNO-9730414 MITF-M-regulated melanocyte development
Reactome:R-DME-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-DME-1266738 Developmental Biology
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-RNO-1266738 Developmental Biology
Reactome:R-BTA-1266738 Developmental Biology
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-72766 Translation
Reactome:R-MMU-1266738 Developmental Biology
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2408522 Selenoamino acid metabolism
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0121283 tRNA Charging 2
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0119383 Protein Synthesis: Arginine
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0121281 tRNA Charging
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0111853 Protein Synthesis: Arginine
PathBank:SMP0119307 Protein Synthesis: Arginine
PathBank:SMP0119463 Protein Synthesis: Arginine
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0087178 Arginine and Proline Metabolism