EC: 6.1.1.10

methionine-tRNA ligase (L-methionine:tRNAMet ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q5UR82 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + L_Methionine + tRNA(Met) -> L_Methionyl_tRNA(Met) + Diphosphate + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Na-Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.5
Temperature: 30
uniprot:Q5UR82 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(Met) + L_Methionine -> AMP + L_Methionyl_tRNA(Met) + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Na-Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.5
Temperature: 30
uniprot:Q5UR82 [methionine---tRNA ligase(Enzyme) wildtype]

tRNA(Met) + L_Methionine + ATP -> AMP + Diphosphate + L_Methionyl_tRNA(Met)		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Na-Hepes, 10 mM MgCl2, 1 mM dithioerythritol
PH: 7.5
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + L_Methionine + tRNA(Met) -> Diphosphate + L_Methionyl_tRNA(Met) + AMP		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + L_Methionine + tRNA(Met) -> Diphosphate + L_Methionyl_tRNA(Met) + AMP		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

tRNA(Met) + L_Methionine + ATP -> AMP + Diphosphate + L_Methionyl_tRNA(Met)		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + L_Methionine + tRNA(Met) -> AMP + L_Methionyl_tRNA(Met) + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(Met) + L_Methionine -> AMP + L_Methionyl_tRNA(Met) + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

tRNA(Met) + L_Methionine + ATP -> L_Methionyl_tRNA(Met) + AMP + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + L_Methionine + tRNA(Met) -> Diphosphate + L_Methionyl_tRNA(Met) + AMP		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

tRNA(Met) + L_Methionine + ATP -> L_Methionyl_tRNA(Met) + AMP + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

tRNA(Met) + ATP + L_Methionine -> AMP + Diphosphate + L_Methionyl_tRNA(Met)		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + L_Methionine + tRNA(Met) -> AMP + L_Methionyl_tRNA(Met) + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

tRNA(Met) + ATP + L_Methionine -> AMP + L_Methionyl_tRNA(Met) + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30
uniprot:Q837B3 [methionine---tRNA ligase(Enzyme) wildtype]

ATP + L_Methionine + tRNA(Met) -> L_Methionyl_tRNA(Met) + AMP + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 0.1 mM Tris-chloride, 15 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.6
Temperature: 30

Pathways

pathway id name
BioCyc:ANTHRA_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULONA1000_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
Reactome:R-DDI-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-DDI-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-DDI-1266738 Developmental Biology
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-72766 Translation
Reactome:R-MMU-1266738 Developmental Biology
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2408522 Selenoamino acid metabolism
Reactome:R-HSA-379724 tRNA Aminoacylation
WikiPathways:WP4288 MTHFR deficiency
PathBank:SMP0000177 Cystathionine beta-Synthase Deficiency
PathBank:SMP0000340 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0087161 Selenoamino Acid Metabolism
PathBank:SMP0120471 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120487 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120519 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120527 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0121283 tRNA Charging 2
PathBank:SMP0000033 Methionine Metabolism
PathBank:SMP0000222 Glycine N-Methyltransferase Deficiency
PathBank:SMP0087249 Methionine Metabolism
PathBank:SMP0063587 Selenoamino Acid Metabolism
PathBank:SMP0063637 Methionine Metabolism
PathBank:SMP0111875 Protein Synthesis: Methionine
PathBank:SMP0119318 Protein Synthesis: Methionine
PathBank:SMP0119405 Protein Synthesis: Methionine
PathBank:SMP0120634 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0121281 tRNA Charging
PathBank:SMP0000341 Hypermethioninemia
PathBank:SMP0000221 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120498 Hypermethioninemia
PathBank:SMP0000029 Selenoamino Acid Metabolism
PathBank:SMP0000214 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000570 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0002371 Selenocompound Metabolism
PathBank:SMP0087423 Selenoamino Acid Metabolism
PathBank:SMP0087483 Methionine Metabolism