EC: 6.1.1.1

tyrosine-tRNA ligase (L-tyrosine:tRNATyr ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

tRNA(Tyr) + L_Tyrosine + ATP -> AMP + Diphosphate + L_Tyrosyl_tRNA(Tyr)
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(Tyr) + L_Tyrosine -> L_Tyrosyl_tRNA(Tyr) + Diphosphate + AMP
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

tRNA(Tyr) + L_Tyrosine + ATP -> L_Tyrosyl_tRNA(Tyr) + Diphosphate + AMP
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

ATP + L_Tyrosine + tRNA(Tyr) -> L_Tyrosyl_tRNA(Tyr) + Diphosphate + AMP
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

ATP + L_Tyrosine + tRNA(Tyr) -> L_Tyrosyl_tRNA(Tyr) + AMP + Diphosphate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

tRNA(Tyr) + ATP + L_Tyrosine -> Diphosphate + L_Tyrosyl_tRNA(Tyr) + AMP
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

tRNA(Tyr) + ATP + L_Tyrosine -> AMP + L_Tyrosyl_tRNA(Tyr) + Diphosphate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(Tyr) + L_Tyrosine -> AMP + L_Tyrosyl_tRNA(Tyr) + Diphosphate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(Tyr) + L_Tyrosine -> AMP + Diphosphate + L_Tyrosyl_tRNA(Tyr)
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

ATP + L_Tyrosine + tRNA(Tyr) -> L_Tyrosyl_tRNA(Tyr) + Diphosphate + AMP
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:Q5UPJ7 [tyrosine---tRNA ligase(Enzyme) wildtype]

tRNA(Tyr) + L_Tyrosine + ATP -> Diphosphate + L_Tyrosyl_tRNA(Tyr) + AMP
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Na-Hepes, 12 mM MgCl2, 25 mM KCl, 0.2 mg/ml BSA, 1 mM spermine
PH: 7.5
Temperature: 30
uniprot:P0AGJ9 [tyrosine---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(Tyr) + L_Tyrosine -> AMP + L_Tyrosyl_tRNA(Tyr) + Diphosphate
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 40 mM Tris- HCl, 1.6 mM ATP, 3.2 mM KCl, 0.8 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0AGJ9 [tyrosine---tRNA ligase(Enzyme) mutant F130S]

tRNA(Tyr) + ATP + L_Tyrosine -> L_Tyrosyl_tRNA(Tyr) + AMP + Diphosphate
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 40 mM Tris- HCl, 1.6 mM ATP, 3.2 mM KCl, 0.8 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0AGJ9 [tyrosine---tRNA ligase(Enzyme) wildtype]

L_beta_(5_Hydroxy_2_pyridyl)_alanine + ATP + tRNA(Tyr) -> L_beta_(5_Hydroxy_2_pyridyl)_alanyl_tRNATyr + AMP + Diphosphate
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 40 mM Tris- HCl, 1.6 mM ATP, 3.2 mM KCl, 0.8 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0AGJ9 [tyrosine---tRNA ligase(Enzyme) mutant F130S]

tRNA(Tyr) + ATP + L_beta_(5_Hydroxy_2_pyridyl)_alanine -> AMP + L_beta_(5_Hydroxy_2_pyridyl)_alanyl_tRNATyr + Diphosphate
(( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 40 mM Tris- HCl, 1.6 mM ATP, 3.2 mM KCl, 0.8 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q57834 [tyrosine---tRNA ligase(Enzyme) wildtype]

tRNA(Tyr) + ATP + L_Tyrosine -> Diphosphate + AMP + L_Tyrosyl_tRNA(Tyr)
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 1 mM 1,4-dithiothreitol, 10 mM MgCl2
PH: 7.5
Temperature: 45
uniprot:Q57834 [tyrosine---tRNA ligase(Enzyme) wildtype]

L_Tyrosine + ATP + tRNA(Tyr) -> L_Tyrosyl_tRNA(Tyr) + Diphosphate + AMP
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 1 mM 1,4-dithiothreitol, 10 mM MgCl2
PH: 7.5
Temperature: 45
uniprot:Q57834 [tyrosine---tRNA ligase(Enzyme) wildtype]

L_Tyrosine + ATP + tRNA(Tyr)_minihelix -> AMP + L_Tyrosyl_tRNA(Tyr)_minihelix + Diphosphate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 1 mM 1,4-dithiothreitol, 10 mM MgCl2
PH: 7.5
Temperature: 45

Pathways

pathway id name
BioCyc:VCHO_TRNA-CHARGING-PWY tRNA charging
BioCyc:ANTHRA_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULONA1000_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-72766 Translation
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0120540 Phenylketonuria
PathBank:SMP0000369 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0120733 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0119304 Protein Synthesis: Tyrosine
PathBank:SMP0119324 Protein Synthesis: Tyrosine
PathBank:SMP0119480 Protein Synthesis: Tyrosine
PathBank:SMP0120511 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0000206 Phenylketonuria
PathBank:SMP0087313 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0120760 Phenylketonuria
PathBank:SMP0120513 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0000370 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0120731 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0000008 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0063649 Phenylalanine and Tyrosine Metabolism