EC: 5.6.2.2

DNA topoisomerase (ATP-hydrolysing) (DNA topoisomerase (ATP-hydrolysing))

enzyme lambda metabolic reaction experiment
uniprot:P15348 [Mg2+]

ATP + negatively_supercoiled_circular_DNA -> ADP + Phosphate + relaxed_circular_DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM Tris-Cl, 50 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 15 µg/ml bovine serum albumin
PH: 7.9
Temperature: 30
uniprot:P15348 [Mg2+]

ATP + negatively_supercoiled_circular_DNA -> Phosphate + relaxed_circular_DNA + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM Tris-Cl, 50 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 15 µg/ml bovine serum albumin
PH: 7.9
Temperature: 30
uniprot:P15348 [Mg2+]

ATP + negatively_supercoiled_circular_DNA -> Phosphate + relaxed_circular_DNA + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Tris-Cl, 50 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 15 µg/ml bovine serum albumin
PH: 7.9
Temperature: 30
uniprot:P15348 [Mg2+]

ATP + negatively_supercoiled_circular_DNA -> relaxed_circular_DNA + ADP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Tris-Cl, 50 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 15 µg/ml bovine serum albumin
PH: 7.9
Temperature: 30
uniprot:P15348 [Mg2+]

dATP + negatively_supercoiled_circular_DNA -> Phosphate + dADP + relaxed_circular_DNA		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Tris-Cl, 50 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 15 µg/ml bovine serum albumin
PH: 7.9
Temperature: 30
uniprot:P15348 [Mg2+]

ATP + negatively_supercoiled_circular_DNA -> Phosphate + ADP + relaxed_circular_DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM Tris-Cl, 50 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 15 µg/ml bovine serum albumin
PH: 7.9
Temperature: 30
uniprot:P15348 [Mg2+]

ATP + positively_supercoiled_linear_DNA -> Phosphate + relaxed_linear_DNA + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Tris, 100 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 0.2% Tween-20, 0.5 mM DTT, 200 µg/ml BSA
PH: 8
Temperature: 25

Pathways

pathway id name
WikiPathways:WP2472 Escherichia coli K-12 peripherome
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-HSA-9638771 Action of antimicrobials
Reactome:R-HSA-9640148 Infection with Enterobacteria
Reactome:R-HSA-9638737 Action of antimicrobials and antimicrobial resistance
Reactome:R-HSA-9913143 Antimicrobial resistance
Reactome:R-CEL-2990846 SUMOylation
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DDI-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-DME-597592 Post-translational protein modification
Reactome:R-DME-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-HSA-1474165 Reproduction
Reactome:R-HSA-912446 Meiotic recombination
Reactome:R-HSA-1362277 Transcription of E2F targets under negative control by DREAM complex
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-2990846 SUMOylation
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-4615885 SUMOylation of DNA replication proteins
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-2990846 SUMOylation
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-SCE-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-CEL-392499 Metabolism of proteins
Reactome:R-CEL-597592 Post-translational protein modification
Reactome:R-CEL-4615885 SUMOylation of DNA replication proteins
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-2990846 SUMOylation
Reactome:R-HSA-1500620 Meiosis
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-HSA-1538133 G0 and Early G1
Reactome:R-HSA-4615885 SUMOylation of DNA replication proteins
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-4615885 SUMOylation of DNA replication proteins
Reactome:R-SCE-2990846 SUMOylation
Reactome:R-SPO-4615885 SUMOylation of DNA replication proteins
Reactome:R-CEL-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-DME-392499 Metabolism of proteins
Reactome:R-DME-2990846 SUMOylation
Reactome:R-DME-4615885 SUMOylation of DNA replication proteins
Reactome:R-HSA-69278 Cell Cycle, Mitotic
Reactome:R-HSA-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-2990846 SUMOylation
Reactome:R-RNO-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-SCE-4615885 SUMOylation of DNA replication proteins
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-SPO-2990846 SUMOylation
Reactome:R-DDI-4615885 SUMOylation of DNA replication proteins
Reactome:R-HSA-453279 Mitotic G1 phase and G1/S transition
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-3108232 SUMO E3 ligases SUMOylate target proteins
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SPO-597592 Post-translational protein modification
Reactome:R-SPO-3108232 SUMO E3 ligases SUMOylate target proteins
PathBank:SMP0000601 Etoposide Metabolism Pathway
PathBank:SMP0063772 Apoptotic DNA Fragmentation and Tissue Homeostasis
PathBank:SMP0108234 Apoptotic DNA Fragmentation and Tissue Homeostasis
PathBank:SMP0000442 Etoposide Action Pathway
PathBank:SMP0000602 Teniposide Metabolism Pathway
PathBank:SMP0000443 Teniposide Action Pathway