EC: 4.3.1.3

histidine ammonia-lyase (L-histidine ammonia-lyase (urocanate-forming))

enzyme lambda metabolic reaction experiment
- [histidine ammonia-lyase(Enzyme) wildtype]

Histidine -> Urocanate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 1 mM EDTA, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

Histidine -> Urocanate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 1 mM EDTA, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

Histidine -> NH3 + Urocanate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 0.1 mM CdCl2, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

Histidine -> Urocanate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 0.1 mM CdCl2, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [4-Fluoro-L-histidine]

Histidine -> Urocanate + NH3		 (( (Vmax * A) ) / (A + (Km * (1 + (I / Kis))))) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 0.1 mM MnCl2
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

NH3 + Urocanate -> Histidine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 1 mM EDTA, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

4_Fluorohistidine -> 4_Fluorourocanate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 1 mM EDTA, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

4_Fluorohistidine -> 4_Fluorourocanate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 0.1 mM CdCl2, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

4_Fluorohistidine -> 4_Fluorourocanate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 0.1 mM CdCl2, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

4_Fluorohistidine -> NH3 + 4_Fluorourocanate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 1 mM EDTA, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25
- [histidine ammonia-lyase(Enzyme) wildtype]

4_Fluorourocanate + NH3 -> 4_Fluorohistidine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-NaOH, 1 mM EDTA, 1 mM Mercaptoethylamine
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-5028 L-histidine degradation II
BioCyc:META_PWY-5030 L-histidine degradation III
BioCyc:META_HISHP-PWY L-histidine degradation VI
BioCyc:VCHO_HISDEG-PWY L-histidine degradation I
BioCyc:ANTHRA_HISDEG-PWY L-histidine degradation I
BioCyc:BSUB_HISDEG-PWY L-histidine degradation I
BioCyc:META_HISDEG-PWY L-histidine degradation I
BioCyc:CAULONA1000_PWY-5028 histidine degradation II
BioCyc:GCF_000013425_HISDEG-PWY L-histidine degradation I
BioCyc:HUMAN_PWY-5030 histidine degradation
BioCyc:CAULO_HISDEG-PWY histidine degradation I
BioCyc:AGRO_PWY-5028 L-histidine degradation II
BioCyc:MOUSE_PWY-5030 histidine degradation III
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-CEL-70921 Histidine catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-HSA-70921 Histidine catabolism
Reactome:R-MMU-70921 Histidine catabolism
Reactome:R-RNO-70921 Histidine catabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-DDI-70921 Histidine catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-BTA-70921 Histidine catabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0000009 Ammonia Recycling
PathBank:SMP0087177 Ammonia Recycling
PathBank:SMP0063579 Ammonia Recycling
PathBank:SMP0120493 Histidinemia
PathBank:SMP0000044 Histidine Metabolism
PathBank:SMP0000191 Histidinemia
PathBank:SMP0087219 Histidine Metabolism
PathBank:SMP0063632 Histidine Metabolism
PathBank:SMP0087431 Ammonia Recycling