EC: 1.13.11.20

cysteine dioxygenase (L-cysteine:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:O50490 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ks))))) buffer: 62.5 mM MES or Tris, 12.5 µM bathocuproine disulfonate
PH: 6.1
Temperature: 37
uniprot:Q9KZL0 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ks))))) buffer: 62.5 mM MES or Tris, 12.5 µM bathocuproine disulfonate
PH: 6
Temperature: 37
uniprot:O32085 [Fe2+]

L_Cysteine + O2 -> 3_Sulfino_L_alanine		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ks))))) buffer: 62.5 mM MES or Tris, 12.5 µM bathocuproine disulfonate
PH: 6.2
Temperature: 37
uniprot:P21816 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ks))))) buffer: 62.5 mM MES or Tris, 12.5 µM bathocuproine disulfonate
PH: 6.1
Temperature: 37
uniprot:Q81CX4 [Fe2+]

L_Cysteine + O2 -> 3_Sulfino_L_alanine		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ks))))) buffer: 62.5 mM MES or Tris, 12.5 µM bathocuproine disulfonate
PH: 6.1
Temperature: 37
uniprot:P21816 [Cysteamine]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM glycine, 300 µg/ml BSA, 10 µg/ml Protein A
PH: 9
Temperature: 37
uniprot:P21816 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM glycine, 300 µg/ml BSA, 10 µg/ml Protein A
PH: 9
Temperature: 37
uniprot:P21816 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM glycine, 300 µg/ml BSA, 10 µg/ml Protein A
PH: 9
Temperature: 37
uniprot:P21816 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM glycine, 300 µg/ml BSA, 10 µg/ml Protein A
PH: 9
Temperature: 37
uniprot:P21816 [D-Cysteine]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM glycine, 300 µg/ml BSA, 10 µg/ml Protein A
PH: 9
Temperature: 37
uniprot:P21816 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM glycine, 300 µg/ml BSA
PH: 9
Temperature: 37
uniprot:P21816 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM glycine, 300 µg/ml BSA, 10 µg/ml Protein A
PH: 9
Temperature: 37
uniprot:Q1G3U6 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25
uniprot:Q9LXG9 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25
uniprot:Q8LGJ5 [Fe2+]

L_Cysteine + O2 -> 3_Sulfino_L_alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25
uniprot:Q9LXT4 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25
uniprot:Q9LXG9 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25
uniprot:Q8LGJ5 [Fe2+]

L_Cysteine + O2 -> 3_Sulfino_L_alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25
uniprot:Q1G3U6 [Fe2+]

L_Cysteine + O2 -> 3_Sulfino_L_alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25
uniprot:Q9SJI9 [Fe2+]

O2 + L_Cysteine -> 3_Sulfino_L_alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis tris propane, 50 mM NaCl, 5 mM TCEP
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-5328 superpathway of methionine degradation
BioCyc:META_CYSTEINE-DEG-PWY L-cysteine degradation I
BioCyc:META_PWY-5328 superpathway of L-methionine salvage and degradation
BioCyc:MOUSE_CYSTEINE-DEG-PWY L-cysteine degradation I
BioCyc:HUMAN_PWY-5331 taurine biosynthesis
BioCyc:MOUSE_PWY-5328 superpathway of methionine degradation
BioCyc:MOUSE_CYSDEGRV-PWY L-cysteine degradation VI
BioCyc:BSUB_CYSTEINE-DEG-PWY L-cysteine degradation I
BioCyc:HUMAN_CYSTEINE-DEG-PWY L-cysteine degradation I
BioCyc:META_PWY-5331 taurine biosynthesis I
Reactome:R-CEL-1614635 Sulfur amino acid metabolism
Reactome:R-HSA-1614558 Degradation of cysteine and homocysteine
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-1614558 Degradation of cysteine and homocysteine
Reactome:R-RNO-1614558 Degradation of cysteine and homocysteine
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1614558 Degradation of cysteine and homocysteine
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1614635 Sulfur amino acid metabolism
Reactome:R-MMU-1614635 Sulfur amino acid metabolism
Reactome:R-RNO-1614635 Sulfur amino acid metabolism
WikiPathways:WP5355 Metabolic Epileptic Disorders
WikiPathways:WP4292 Methionine metabolism leading to sulfur amino acids and related disorders
WikiPathways:WP4504 Cysteine and methionine catabolism
PathBank:SMP0000722 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0087189 Cysteine Metabolism
PathBank:SMP0000499 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0000013 Cysteine Metabolism
PathBank:SMP0000021 Taurine and Hypotaurine Metabolism
PathBank:SMP0087214 Taurine and Hypotaurine Metabolism