EC: 1.1.1.153

sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) (L-erythro-7,8-dihydrobiopterin:NADP+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + Sepiapterin + NADPH -> 7,8_Dihydrobiopterin + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Acetoin + NADPH + H+ -> NADP+ + 2,3_Butanediol		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + NADPH + Dimethylglyoxal -> NADP+ + Acetoin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

1_Phenyl_1,2_propanedione + NADPH + H+ -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADPH + H+ + Benzil -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype S46/S196/S214 phosphorylated]

Sepiapterin + NADPH + H+ -> NADP+ + 7,8_Dihydrobiopterin		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADPH + Sepiapterin + H+ -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Sepiapterin + H+ + NADPH -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) mutant S46A/S196A/S214A]

Sepiapterin + NADPH + H+ -> NADP+ + 7,8_Dihydrobiopterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype S46/S196/S214 phosphorylated]

NADPH + H+ + Sepiapterin -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) mutant S46A/S196A/S214A]

H+ + NADPH + Sepiapterin -> NADP+ + 7,8_Dihydrobiopterin		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P35270 [sepiapterin reductase(Enzyme) mutant S213A]

Sepiapterin + NADPH + H+ -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P35270 [sepiapterin reductase(Enzyme) mutant S213A]

Sepiapterin + NADPH + H+ -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P35270 [sepiapterin reductase(Enzyme) wildtype S213 phosphorylated]

NADPH + Sepiapterin + H+ -> NADP+ + 7,8_Dihydrobiopterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P35270 [sepiapterin reductase(Enzyme) wildtype]

NADPH + H+ + Sepiapterin -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P35270 [sepiapterin reductase(Enzyme) wildtype S213 phosphorylated]

H+ + Sepiapterin + NADPH -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P35270 [sepiapterin reductase(Enzyme) wildtype]

Sepiapterin + NADPH + H+ -> 7,8_Dihydrobiopterin + NADP+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM sodium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Methylglyoxal + H+ + NADPH -> Hydroxyacetone + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADPH + Sepiapterin + H+ -> NADP+ + 7,8_Dihydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADH + H+ + Dimethylglyoxal -> Acetoin + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + NADPH + Dimethylglyoxal -> NADP+ + Acetoin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADH + H+ + Sepiapterin -> 7,8_Dihydrobiopterin + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + 1_Phenyl_1,2_propanedione + NADH -> Unknown + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADPH + 1_Phenyl_1,2_propanedione + H+ -> Unknown + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Phenylglyoxal + NADPH + H+ -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADPH + H+ + Benzil -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

p_Nitrobenzaldehyde + NADPH + H+ -> p_Nitrobenzyl_alcohol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + NADPH + 9,10_Phenanthroquinone -> NADP+ + Phenanthrene_9,10_diol		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADPH + Menadione + H+ -> Menadiol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Acetoin + H+ + NADPH -> 2,3_Butanediol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + 1,4_Benzoquinone + NADPH -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Acetophenone + H+ + NADPH -> Unknown + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + NADPH + 2,4_Pentanedione -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

NADPH + H+ + Propiophenone -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + Benzylacetone + NADPH -> NADP+ + Unknown		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + Sepiapterin + NADPH -> 7,8_Dihydrobiopterin + NADP+		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Dimethylglyoxal + NADH + H+ -> Acetoin + NAD+		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

Dimethylglyoxal + H+ + NADPH -> NADP+ + Acetoin		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + NADH + Sepiapterin -> NAD+ + 7,8_Dihydrobiopterin		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + NADH + 1_Phenyl_1,2_propanedione -> NAD+ + Unknown		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25
uniprot:P18297 [sepiapterin reductase(Enzyme) wildtype]

H+ + NADPH + 1_Phenyl_1,2_propanedione -> NADP+ + Unknown		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM potassium phosphate
PH: 6.4
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-5664 erythro-tetrahydrobiopterin biosynthesis II
BioCyc:HUMAN_PWY-5663 tetrahydrobiopterin de novo biosynthesis
BioCyc:MOUSE_PWY-5663 tetrahydrobiopterin biosynthesis I
BioCyc:META_PWY-5663 erythro-tetrahydrobiopterin biosynthesis I
BioCyc:MOUSE_PWY-5664 tetrahydrobiopterin biosynthesis II
Reactome:R-HSA-202131 Metabolism of nitric oxide: NOS3 activation and regulation
Reactome:R-HSA-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-202131 Metabolism of nitric oxide: NOS3 activation and regulation
Reactome:R-MMU-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
Reactome:R-RNO-202131 Metabolism of nitric oxide: NOS3 activation and regulation
Reactome:R-RNO-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
Reactome:R-DDI-202131 Metabolism of nitric oxide: NOS3 activation and regulation
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-8978934 Metabolism of cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-203615 eNOS activation
Reactome:R-HSA-8978934 Metabolism of cofactors
Reactome:R-MMU-8978934 Metabolism of cofactors
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-8978934 Metabolism of cofactors
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-203615 eNOS activation
Reactome:R-MMU-203615 eNOS activation
Reactome:R-RNO-203615 eNOS activation
WikiPathways:WP4156 Biosynthesis and regeneration of tetrahydrobiopterin and catabolism of phenylalanine
PathBank:SMP0120774 Hyperphenylalaninemia Due to DHPR-Deficiency
PathBank:SMP0120552 Hyperphenylalaninemia Due to Guanosine Triphosphate Cyclohydrolase Deficiency
PathBank:SMP0120556 Sepiapterin Reductase Deficiency
PathBank:SMP0000005 Pterine Biosynthesis
PathBank:SMP0000488 Hyperphenylalaninemia Due to 6-Pyruvoyltetrahydropterin Synthase Deficiency (ptps)
PathBank:SMP0120773 Hyperphenylalaninemia Due to 6-Pyruvoyltetrahydropterin Synthase Deficiency (ptps)
PathBank:SMP0120551 DOPA-Responsive Dystonia
PathBank:SMP0120555 Segawa Syndrome
PathBank:SMP0000487 Hyperphenylalaninemia Due to Guanosine Triphosphate Cyclohydrolase Deficiency
PathBank:SMP0000491 Sepiapterin Reductase Deficiency
PathBank:SMP0120772 Hyperphenylalaninemia Due to Guanosine Triphosphate Cyclohydrolase Deficiency
PathBank:SMP0120776 Sepiapterin Reductase Deficiency
PathBank:SMP0120553 Hyperphenylalaninemia Due to 6-Pyruvoyltetrahydropterin Synthase Deficiency (ptps)
PathBank:SMP0000489 Hyperphenylalaninemia Due to DHPR-Deficiency
PathBank:SMP0120771 DOPA-Responsive Dystonia
PathBank:SMP0120775 Segawa Syndrome
PathBank:SMP0120554 Hyperphenylalaninemia Due to DHPR-Deficiency
PathBank:SMP0000486 DOPA-Responsive Dystonia
PathBank:SMP0000490 Segawa Syndrome
PathBank:SMP0087343 Pterine Biosynthesis
PathBank:SMP0063657 Pterine Biosynthesis