EC: 3.5.3.7

guanidinobutyrase (4-guanidinobutanoate amidinohydrolase)

enzyme lambda metabolic reaction experiment
- [guanidinobutyrase(Enzyme) wildtype isoenzym II]

H2O + L_Arginine -> Urea + L_Ornithine
(( (Vmax * S) ) / (Km + S)) buffer: 25 mM glycine/NaOH, 5 mM MnCl2
PH: 9.5
Temperature: 30
- [L-Arginine]

H2O + 4_Guanidinobutanoate -> 4_Aminobutanoate + Urea
(( (Vmax * S) ) / (Km + S)) buffer: 25 mM glycine/NaOH, 5 mM MnCl2
PH: 9.5
Temperature: 30
- [guanidinobutyrase(Enzyme) wildtype isoenzym II]

4_Guanidinobutanoate + H2O -> Urea + 4_Aminobutanoate
(( (Vmax * S) ) / (Km + S)) buffer: 25 mM glycine/NaOH, 5 mM MnCl2
PH: 9.5
Temperature: 30
- [guanidinobutyrase(Enzyme) wildtype isoenzym II]

H2O + 3_Guanidinopropanoate -> beta_Alanine + Urea
(( (Vmax * S) ) / (Km + S)) buffer: 25 mM glycine/NaOH, 5 mM MnCl2
PH: 9.5
Temperature: 30
- [guanidinobutyrase(Enzyme) wildtype isoenzym II]

Canavanine + H2O -> L_Canaline + Urea
(( (Vmax * S) ) / (Km + S)) buffer: 25 mM glycine/NaOH, 5 mM MnCl2
PH: 9.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-40 putrescine biosynthesis I
BioCyc:META_PWY-5742 L-arginine degradation IX (arginine:pyruvate transaminase pathway)
BioCyc:MOUSE_PWY0-823 arginine degradation III (arginine decarboxylase/agmatinase pathway)
BioCyc:MOUSE_PWY-40 putrescine biosynthesis I
BioCyc:META_ARGDEG-V-PWY L-arginine degradation X (arginine monooxygenase pathway)
BioCyc:HUMAN_PWY-40 putrescine biosynthesis II
BioCyc:META_PWY-7523 L-arginine degradation XII
Reactome:R-HSA-351202 Metabolism of polyamines
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-351202 Metabolism of polyamines
Reactome:R-RNO-351202 Metabolism of polyamines
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-351143 Agmatine biosynthesis
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-351143 Agmatine biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-RNO-351143 Agmatine biosynthesis
Reactome:R-HSA-1430728 Metabolism