EC: 3.5.2.3

dihydroorotase ((S)-dihydroorotate amidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> L_Dihydroorotate + H2O		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MES
PH: 5.8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MES
PH: 5.8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

L_Dihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

L_Dihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

L_6_Thiodihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) mutant R20K]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MES
PH: 5.8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> L_Dihydroorotate + H2O		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MES
PH: 5.8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) mutant D250S]

N_Carbamoyl_L_aspartate -> L_Dihydroorotate + H2O		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MES
PH: 5.8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) mutant D250E]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MES
PH: 5.8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

L_Dihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 8
Temperature: 36
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

L_6_Thiodihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (( (Et * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 8
Temperature: 36
uniprot:O66990 [dihydroorotase(Enzyme) wildtype]

L_Dihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris acetate, 10% glycerol, stoichiometric amounts of glutaminase domain, carbamoyl-phosphate synthetase subunit A, carbamoyl-phosphate synthetase subunit B, aspartate transcarbamoylase
PH: 8.3
Temperature: 75
- [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (Vmax * A) ) / (Km + A)) buffer: 66.6 mM Mes/NaOH
PH: 5.3
Temperature: 36
- [dihydroorotase(Enzyme) wildtype zinc reconstituted]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (Vmax * A) ) / (Km + A)) buffer: 66.6 mM Mes/NaOH
PH: 5.3
Temperature: 36
- [dihydroorotase(Enzyme) wildtype]

L_Dihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris/acetate
PH: 8
Temperature: 36
- [dihydroorotase(Enzyme) wildtype zinc reconstituted]

H2O + L_Dihydroorotate -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris/acetate
PH: 8
Temperature: 36
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

H2O + Dihydroorotate -> N_Carbamoyl_DL_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium HEPES
PH: 7.33
Temperature: 37
uniprot:P08955 [dihydroorotase(Enzyme) wildtype DHO domain]

N_Carbamoyl_L_aspartate -> L_Dihydroorotate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.10 M Tris chloride, 0.10 M KCl, 3.3 mM glutamine, 15 mM L-aspartate, 25 mM MgCl2, 7.5% (v/v) dimethyl sulfoxide, 2.5% (w/v) glycerol, 3 mM dithiothreitol
PH: 7.7
Temperature: 37
uniprot:P08955 [dihydroorotase(Enzyme) wildtype DHO domain]

H2O + L_Dihydroorotate -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.10 M Tris chloride, 0.10 M KCl, 3.3 mM glutamine, 15 mM L-aspartate, 25 mM MgCl2, 7.5% (v/v) dimethyl sulfoxide, 2.5% (w/v) glycerol, 3 mM dithiothreitol
PH: 7.7
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium HEPES
PH: 7.27
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

H2O + L_Dihydroorotate -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium HEPES
PH: 7.27
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium HEPES
PH: 7
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium Mes
PH: 6
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> L_Dihydroorotate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8.3
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

L_Dihydroorotate + H2O -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium Mes
PH: 5
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

H2O + L_Dihydroorotate -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium HEPES
PH: 7.3
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium HEPES
PH: 7.33
Temperature: 37
uniprot:B2RQC6 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_D_aspartate -> D_Dihydroorotate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium HEPES
PH: 7.33
Temperature: 37
uniprot:P08955 [dihydroorotase(Enzyme) wildtype CAD]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M Tris chloride, 0.2 M KCl, 15% dimethyl sulfoxide, 5% glycerol, 2 mM dithiothreitol
PH: 7.7
Temperature: 37
uniprot:P08955 [dihydroorotase(Enzyme) wildtype CAD]

H2O + L_Dihydroorotate -> N_Carbamoyl_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M Tris chloride, 0.2 M KCl, 15% dimethyl sulfoxide, 5% glycerol, 2 mM dithiothreitol
PH: 7.7
Temperature: 37
uniprot:P05020 [dihydroorotase(Enzyme) mutant T110S]

N_Carbamoyl_L_aspartate -> L_Dihydroorotate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 5.8
Temperature: 30
uniprot:P05020 [dihydroorotase(Enzyme) mutant T110A]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 5.8
Temperature: 30
uniprot:P05020 [dihydroorotase(Enzyme) mutant T110V]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 5.8
Temperature: 30
uniprot:P05020 [dihydroorotase(Enzyme) wildtype]

N_Carbamoyl_L_aspartate -> L_Dihydroorotate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 5.8
Temperature: 30
uniprot:P05020 [dihydroorotase(Enzyme) mutant T109S]

N_Carbamoyl_L_aspartate -> H2O + L_Dihydroorotate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 5.8
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY-5686 UMP biosynthesis
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:BSUB_PWY-7791 UMP biosynthesis III
BioCyc:CORYNE_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:META_PWY-5686 UMP biosynthesis I
BioCyc:ECO_PWY-5686 UMP biosynthesis I
BioCyc:ARA_PWY-5686 UMP biosynthesis I
BioCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY-5686 UMP biosynthesis I
BioCyc:MTBH37RV_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY-5686 UMP biosynthesis I
BioCyc:ECOO157_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-5686 UMP biosynthesis
BioCyc:ECOL199310_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY-5686-1 UMP biosynthesis
BioCyc:ECOL316407_PWY-5686 UMP biosynthesis
BioCyc:PDIF272563_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:CORYNE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7790 UMP biosynthesis II
BioCyc:META_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ECO_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-7790 UMP biosynthesis II
BioCyc:ARA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-5686 UMP biosynthesis
BioCyc:BSUB_PWY-5686-1 uridine-5'-phosphate biosynthesis
BioCyc:ECOL316407_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:META_PWY-7791 UMP biosynthesis III
BioCyc:ECO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:YEAST_PYRIMID-RNTSYN-PWY de novo biosynthesis of pyrimidine ribonucleotides
BioCyc:ECOO157_PWY-5686 UMP biosynthesis
BioCyc:ECOL199310_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:PDIF272563_PWY-5686 UMP biosynthesis I
BioCyc:PDIF272563_PWY-5686-1 UMP biosynthesis II
BioCyc:CORYNE_PWY-5686 UMP biosynthesis
PathBank:SMP0000959 Pyrimidine Metabolism
PlantCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY-5686 UMP biosynthesis I
PlantCyc:PLANT_PWY-5686 UMP biosynthesis I
PlantCyc:PLANT_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
Reactome:R-CEL-500753 Pyrimidine biosynthesis
Reactome:R-DDI-8956320 Nucleotide biosynthesis
Reactome:R-DME-500753 Pyrimidine biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-8956320 Nucleotide biosynthesis
Reactome:R-MMU-8956320 Nucleotide biosynthesis
Reactome:R-CEL-15869 Nucleotide metabolism
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-HSA-500753 Pyrimidine biosynthesis
Reactome:R-MMU-500753 Pyrimidine biosynthesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-8956320 Nucleotide biosynthesis
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-500753 Pyrimidine biosynthesis
Reactome:R-DME-8956320 Nucleotide biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
PathBank:SMP0108157 Apoptotic DNA Fragmentation and Tissue Homeostasis
PathBank:SMP0120456 Argininosuccinic Aciduria
PathBank:SMP0120488 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120504 Lactic Acidemia
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0120613 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0120637 Glycogenosis, Type IB
PathBank:SMP0120645 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0121324 Pyrimidine Metabolism
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0087426 Aspartate Metabolism
PathBank:SMP0063579 Ammonia Recycling
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0063661 Pyruvate Metabolism
PathBank:SMP0108760 Glutaminolysis and Cancer
PathBank:SMP0120455 Argininemia
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120507 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0120531 Pyruvate Dehydrogenase Complex Deficiency
PathBank:SMP0120535 Pyruvate Carboxylase Deficiency
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120543 Primary Hyperoxaluria Type I
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120612 Mitochondrial Complex II Deficiency
PathBank:SMP0120624 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0087473 Pyrimidine Metabolism
PathBank:SMP0063617 Gluconeogenesis
PathBank:SMP0063772 Apoptotic DNA Fragmentation and Tissue Homeostasis
PathBank:SMP0108758 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120461 Canavan Disease
PathBank:SMP0120473 Dihydropyrimidinase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120533 Pyruvate Decarboxylase E1 Component Deficiency (PDHE1 Deficiency)
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120610 Congenital Lactic Acidosis
PathBank:SMP0120614 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0120622 Primary Hyperoxaluria II, PH2
PathBank:SMP0120626 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0120638 Glycogenosis, Type IC
PathBank:SMP0000067 Aspartate Metabolism
PathBank:SMP0000175 Canavan Disease
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0002370 Pyrimidine Metabolism
PathBank:SMP0087428 Glutamate Metabolism
PathBank:SMP0063586 Alanine Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0063658 Pyrimidine Metabolism
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120458 beta-Ureidopropionase Deficiency
PathBank:SMP0120462 Carbamoyl Phosphate Synthetase Deficiency
PathBank:SMP0120466 Citrullinemia Type I
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120502 Hypoacetylaspartia
PathBank:SMP0120506 Leigh Syndrome
PathBank:SMP0120530 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120542 Ornithine Transcarbamylase Deficiency (OTC Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120611 Fumarase Deficiency
PathBank:SMP0120615 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0120623 Pyruvate Kinase Deficiency
PathBank:SMP0120627 Triosephosphate Isomerase Deficiency
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000192 Hypoacetylaspartia
PathBank:SMP0000172 beta-Ureidopropionase Deficiency
PathBank:SMP0086930 Warburg Effect
PathBank:SMP0087431 Ammonia Recycling
PathBank:SMP0063477 Citric Acid Cycle
PathBank:SMP0063597 Aspartate Metabolism
PathBank:SMP0063681 Transfer of Acetyl Groups into Mitochondria
PathBank:SMP0063686 Urea Cycle