EC: 3.5.1.9

arylformamidase (aryl-formylamine amidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P0C8P4 [arylformamidase(Enzyme) wildtype N-terminal His-tagged]

L_Formylkynurenine + H2O -> L_Kynurenine + Formate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 20 µM ZnCl2
PH: 7.4
Temperature: 37
uniprot:Q8K4H1 [arylformamidase(Enzyme) wildtype]

H2O + L_Formylkynurenine -> Formate + L_Kynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.4
Temperature: 36
uniprot:Q8K4H1 [arylformamidase(Enzyme) wildtype]

H2O + L_Formylkynurenine -> Formate + L_Kynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate
PH: 7.4
Temperature: 36

Pathways

pathway id name
BioCyc:HUMAN_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY-5651 L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:YEAST_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:MOUSE_TRPCAT-PWY tryptophan degradation I (via anthranilate)
BioCyc:HUMAN_PWY-6309 L-kynurenine degradation
BioCyc:YEAST_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_TRYPTOPHAN-DEGRADATION-1 L-tryptophan degradation III (eukaryotic)
BioCyc:META_PWY-6309 L-tryptophan degradation XI (mammalian, via kynurenine)
BioCyc:MOUSE_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation III (eukaryotic)
BioCyc:MOUSE_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
Reactome:R-HSA-71240 Tryptophan catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP5044 Kynurenine pathway and links to cell senescence
PathBank:SMP0002353 Tryptophan Metabolism
PathBank:SMP0000063 Tryptophan Metabolism
PathBank:SMP0002338 NAD Metabolism