EC: 3.5.1.6

beta-ureidopropionase (N-carbamoyl-beta-alanine amidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:B4FSZ8 [beta-ureidopropionase(Enzyme) wildtype]

H2O + N_Carbamoyl_beta_alanine -> NH3 + beta_Alanine + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.094 M Potassium phosphate, 0.94 mM MgCl2, 1.88 mM Dithiothreitol
PH: 7
Temperature: 25
uniprot:B4FSZ8 [beta-ureidopropionase(Enzyme) wildtype]

3_Ureidoisobutyrate + H2O -> NH3 + 3_Aminoisobutanoate + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.094 M Potassium phosphate, 0.94 mM MgCl2, 1.88 mM Dithiothreitol
PH: 7
Temperature: 25
uniprot:Q03248 [beta-ureidopropionase(Enzyme) wildtype]

N_Carbamoyl_beta_alanine + H2O -> beta_Alanine + NH3 + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 150 mM Tris
PH: 7
Temperature: 38
uniprot:Q03248 [beta-ureidopropionase(Enzyme) wildtype]

N_Carbamoyl_beta_aminoisobutyrate + H2O -> CO2 + 3_Aminoisobutanoate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 150 mM Tris
PH: 7
Temperature: 38
uniprot:Q03248 [beta-ureidopropionase(Enzyme) wildtype]

H2O + N_Carbamoyl_beta_alanine -> beta_Alanine + CO2 + NH3		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Sodium phosphate, 0.1 % Bovine serum albumin, 10 mM MgCl2, 1 mM EDTA, 5 mM 2-Mercaptoethanol
PH: 7
Temperature: 37
uniprot:Q03248 [beta-ureidopropionase(Enzyme) wildtype]

N_Carbamoyl_beta_aminoisobutyrate + H2O -> CO2 + NH3 + 3_Aminoisobutanoate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Sodium phosphate, 0.1 % Bovine serum albumin, 10 mM MgCl2, 1 mM EDTA, 5 mM 2-Mercaptoethanol
PH: 7
Temperature: 37
uniprot:Q9UBR1 [beta-ureidopropionase(Enzyme) wildtype N-terminal His6-tagged]

H2O + N_Carbamoyl_beta_alanine -> CO2 + beta_Alanine + NH3		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM HEPES, 50 mM NaCl
PH: 7.4
Temperature: 37
uniprot:Q9UBR1 [beta-ureidopropionase(Enzyme) wildtype N-terminal His6-tagged]

H2O + N_Carbamoyl_beta_alanine -> CO2 + beta_Alanine + NH3		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM MES, 50 mM NaCl
PH: 6.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6430 thymine degradation
BioCyc:META_PWY-6430 thymine degradation
BioCyc:META_PWY-3982 uracil degradation I (reductive)
BioCyc:META_PWY-7209 superpathway of pyrimidine ribonucleosides degradation
BioCyc:ARA_PWY-3982 uracil degradation I (reductive)
BioCyc:MOUSE_PWY-3982 uracil degradation II (reductive)
BioCyc:HUMAN_PWY-7209 pyrimidine ribonucleosides degradation
BioCyc:HUMAN_PWY-3982 uracil degradation
PlantCyc:ARA_PWY-3982 uracil degradation I (reductive)
PlantCyc:PLANT_PWY-3982 uracil degradation I (reductive)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DDI-8956319 Nucleotide catabolism
Reactome:R-HSA-73621 Pyrimidine catabolism
Reactome:R-MMU-73621 Pyrimidine catabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-73621 Pyrimidine catabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-73621 Pyrimidine catabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-8956319 Nucleotide catabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-8956319 Nucleotide catabolism
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-8956319 Nucleotide catabolism
WikiPathways:WP4584 Biomarkers for pyrimidine metabolism disorders
WikiPathways:WP4225 Pyrimidine metabolism and related diseases
PathBank:SMP0120678 beta-Ureidopropionase Deficiency
PathBank:SMP0120698 GABA-Transaminase Deficiency
PathBank:SMP0120750 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0120778 Carnosinuria, Carnosinemia
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0000492 Ureidopropionase Deficiency
PathBank:SMP0120693 Dihydropyrimidinase Deficiency
PathBank:SMP0120777 Ureidopropionase Deficiency
PathBank:SMP0120507 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0063599 beta-Alanine Metabolism
PathBank:SMP0120473 Dihydropyrimidinase Deficiency
PathBank:SMP0120558 Carnosinuria, Carnosinemia
PathBank:SMP0000351 GABA-Transaminase Deficiency
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0000493 Carnosinuria, Carnosinemia
PathBank:SMP0087284 beta-Alanine Metabolism
PathBank:SMP0087340 Pyrimidine Metabolism
PathBank:SMP0063658 Pyrimidine Metabolism
PathBank:SMP0120727 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0120458 beta-Ureidopropionase Deficiency
PathBank:SMP0120478 GABA-Transaminase Deficiency
PathBank:SMP0120530 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0000007 beta-Alanine Metabolism
PathBank:SMP0000172 beta-Ureidopropionase Deficiency