EC: 3.2.1.52

beta-N-acetylhexosaminidase (beta-N-acetyl-D-hexosaminide N-acetylhexosaminohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant D119N]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant E121Q]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant E121A]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant D229A]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant D229N]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant D329N]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant E293Q]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant D303N]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q84IZ5 [beta-N-acetylhexosaminidase(Enzyme) mutant D274N]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q22492 [beta-N-acetylhexosaminidase(Enzyme) wildtype]

p_Nitrophenyl_N_acetyl_beta_D_glucosaminide + H2O -> N_Acetyl_beta_D_glucosamine + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: McIlvaine citrate-phosphate
PH: 5.5
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform A]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Sodium citrate, 0.01% Bovine serum albumin
PH: 5
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform B]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Sodium citrate, 0.01% Bovine serum albumin
PH: 5
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase A]

Globoside + H2O -> N_Acetylgalactosamine + N_Acetyl_D_glucosaminyl_1,3_beta_D_galactosyl_1,4_D_glucosylceramide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase A+B]

H2O + GA2 -> beta_D_Galactosyl_1,4_beta_D_glucosylceramide + N_Acetylgalactosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase A]

GA2 + H2O -> beta_D_Galactosyl_1,4_beta_D_glucosylceramide + N_Acetylgalactosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

p_Nitrophenyl_N_acetyl_beta_D_galactosaminide + H2O -> p_Nitrophenol + N_Acetyl_beta_D_galactosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase A+B]

H2O + p_Nitrophenyl_N_acetyl_beta_D_galactosaminide -> p_Nitrophenol + N_Acetyl_beta_D_galactosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

H2O + p_Nitrophenyl_N_acetyl_beta_D_galactosaminide -> p_Nitrophenol + N_Acetyl_beta_D_galactosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase A]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase A+B]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

Globoside + H2O -> N_Acetylgalactosamine + N_Acetyl_D_glucosaminyl_1,3_beta_D_galactosyl_1,4_D_glucosylceramide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

GA2 + H2O -> N_Acetylgalactosamine + beta_D_Galactosyl_1,4_beta_D_glucosylceramide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

H2O + GA2 -> N_Acetylgalactosamine + beta_D_Galactosyl_1,4_beta_D_glucosylceramide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

p_Nitrophenyl_N_acetyl_beta_D_galactosaminide + H2O -> p_Nitrophenol + N_Acetyl_beta_D_galactosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype Hexosaminidase B]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Citrate-phosphate
PH: 3.8
Temperature: 37
uniprot:Q3U4H6 [beta-N-acetylhexosaminidase(Enzyme) wildtype His-tagged]

H2O + p_Nitrophenyl_N_acetyl_beta_D_galactosaminide -> N_Acetyl_beta_D_galactosamine + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: McIlvaine citrate-phosphate, 2.5% (v/v) DMSO
PH: 5.5
Temperature: 37
uniprot:Q7WUL3 [beta-N-acetylhexosaminidase(Enzyme) wildtype His6-tagged]

H2O + p_Nitrophenyl_N_acetyl_beta_D_glucosaminide -> N_Acetyl_beta_D_glucosamine + p_Nitrophenol		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.08
Temperature: 25
uniprot:Q7WUL4 [beta-N-acetylhexosaminidase(Enzyme) wildtype His6-tagged]

H2O + p_Nitrophenyl_N_acetyl_beta_D_glucosaminide -> N_Acetyl_beta_D_glucosamine + p_Nitrophenol		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.08
Temperature: 25
uniprot:Q7WUL4 [beta-N-acetylhexosaminidase(Enzyme) wildtype His6-tagged]

H2O + p_Nitrophenyl_N_acetyl_beta_D_galactosaminide -> N_Acetyl_beta_D_galactosamine + p_Nitrophenol		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.08
Temperature: 25
- [beta-N-acetylhexosaminidase(Enzyme) wildtype]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M KH2PO4, 0.1 M NaOH
PH: 5.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) mutant D232G]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M KH2PO4, 0.1 M NaOH
PH: 5.8
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) mutant H234G]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M KH2PO4, 0.1 M NaOH
PH: 5.8
Temperature: 37
uniprot:P96157 [NaCl]

p_Nitrophenyl_N_acetyl_beta_D_glucosaminide + H2O -> p_Nitrophenol + N_Acetyl_beta_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Hepes
PH: 7
Temperature: 22
- [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform A]

H2O + 4_Methylumbelliferyl_2_acetamido_2_deoxy_beta_D_glucopyranoside -> 4_Methylumbelliferone + 2_Acetamido_2_deoxy_beta_D_glucopyranoside		 (( (Vmax * S) ) / (Km + S)) buffer: 0.15 M NaCl, Citrate/Phosphate, 0.5 mg/ml BSA
PH: 4.5
Temperature: 37
- [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform I2]

4_Methylumbelliferyl_2_acetamido_2_deoxy_beta_D_glucopyranoside + H2O -> 2_Acetamido_2_deoxy_beta_D_glucopyranoside + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.15 M NaCl, Citrate/Phosphate, 0.5 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:P07686 [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform B]

4_Methylumbelliferyl_2_acetamido_2_deoxy_beta_D_glucopyranoside + H2O -> 2_Acetamido_2_deoxy_beta_D_glucopyranoside + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.15 M NaCl, Citrate/Phosphate, 0.5 mg/ml BSA
PH: 4.5
Temperature: 37
uniprot:P06865 [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform S]

H2O + 4_Methylumbelliferyl_beta_N_acetylglucosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_glucosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate phosphate
PH: 4.4
Temperature: 37
uniprot:P06865 [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform S]

4_Methylumbelliferyl_beta_N_acetylglucosaminide + H2O -> N_Acetyl_beta_D_glucosamine + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate phosphate
PH: 4.4
Temperature: 37
uniprot:P06865 [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform S]

H2O + 4_Methylumbelliferyl_beta_N_acetylgalactosaminide -> N_Acetyl_beta_D_galactosamine + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate phosphate
PH: 4.4
Temperature: 37
uniprot:P06865 [beta-N-acetylhexosaminidase(Enzyme) wildtype isoform S]

H2O + 4_Methylumbelliferyl_beta_N_acetylgalactosaminide -> 4_Methylumbelliferone + N_Acetyl_beta_D_galactosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate phosphate
PH: 4.4
Temperature: 37
uniprot:B2UQG6 [beta-N-acetylhexosaminidase(Enzyme) wildtype His6-tagged]

H2O + p_Nitrophenyl_N_acetyl_beta_D_glucosaminide -> p_Nitrophenol + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM NaCl
PH: 7
Temperature: 37
uniprot:B2UQG6 [beta-N-acetylhexosaminidase(Enzyme) mutant D326A His6-tagged]

H2O + p_Nitrophenyl_N_acetyl_beta_D_glucosaminide -> p_Nitrophenol + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM NaCl
PH: 7
Temperature: 37
uniprot:B2UQG6 [beta-N-acetylhexosaminidase(Enzyme) mutant E327A His6-tagged]

p_Nitrophenyl_N_acetyl_beta_D_glucosaminide + H2O -> p_Nitrophenol + N_Acetyl_beta_D_glucosamine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM NaCl
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY0-1261 anhydromuropeptides recycling I
BioCyc:META_PWY-6902 chitin degradation II (Vibrio)
BioCyc:ECOL316407_PWY0-1261 anhydromuropeptides recycling
BioCyc:META_PWY-7883 anhydromuropeptides recycling II
BioCyc:META_PWY-7822 chitin degradation III (Serratia)
BioCyc:VCHO_PWY-6902 chitin degradation II (Vibrio)
BioCyc:ECO_PWY0-1261 anhydromuropeptides recycling I
BioCyc:ECOL199310_PWY0-1261 anhydromuropeptides recycling
PathBank:SMP0000905 Amino Sugar and Nucleotide Sugar Metabolism I
PathBank:SMP0000906 Amino Sugar and Nucleotide Sugar Metabolism II
PathBank:SMP0000914 Amino Sugar and Nucleotide Sugar Metabolism III
PathBank:SMP0002076 1,6-Anhydro-N-acetylmuramic Acid Recycling
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-3560782 Diseases associated with glycosaminoglycan metabolism
Reactome:R-HSA-3781865 Diseases of glycosylation
Reactome:R-HSA-3656234 Defective HEXA causes GM2-gangliosidosis 1
Reactome:R-HSA-5668914 Diseases of metabolism
WikiPathways:WP4153 Degradation pathway of sphingolipids, including diseases
WikiPathways:WP5055 Burn wound healing
PathBank:SMP0120818 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120524 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0122240 1,6-Anhydro-N-acetylmuramic Acid Recycling
PathBank:SMP0000240 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120737 Sialuria or French Type Sialuria
PathBank:SMP0120765 Tay-Sachs Disease
PathBank:SMP0121303 Amino Sugar and Nucleotide Sugar Metabolism III
PathBank:SMP0000045 Amino Sugar Metabolism
PathBank:SMP0000216 Sialuria or French Type Sialuria
PathBank:SMP0087281 Amino Sugar Metabolism
PathBank:SMP0120744 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120517 Sialuria or French Type Sialuria
PathBank:SMP0120545 Tay-Sachs Disease
PathBank:SMP0000390 Tay-Sachs Disease
PathBank:SMP0087175 Amino Sugar Metabolism
PathBank:SMP0120599 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0121302 Amino Sugar and Nucleotide Sugar Metabolism I
PathBank:SMP0000534 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0063580 Amino Sugar Metabolism