EC: 3.2.1.45

glucosylceramidase (D-glucosyl-N-acylsphingosine glucohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q8GAT9 [glucosylceramidase(Enzyme) wildtype His6-tagged]

p_Nitrophenyl_beta_D_glucoside + H2O -> beta_D_Glucose + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Sodium phosphate
PH: 6.5
Temperature: 37
uniprot:Q8GAT9 [glucosylceramidase(Enzyme) wildtype His6-tagged]

H2O + 2,4_Dinitrophenyl_beta_D_glucopyranoside -> beta_D_Glucose + 2,4_Dinitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Sodium phosphate
PH: 6.5
Temperature: 37
uniprot:Q8GAT9 [glucosylceramidase(Enzyme) mutant D223G His6-tagged]

H2O + 2,4_Dinitrophenyl_beta_D_glucopyranoside -> beta_D_Glucose + 2,4_Dinitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Sodium phosphate
PH: 6.5
Temperature: 37
uniprot:Q8GAT9 [glucosylceramidase(Enzyme) mutant E411A His6-tagged]

p_Nitrophenyl_beta_D_glucoside + H2O -> beta_D_Glucose + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Sodium phosphate
PH: 6.5
Temperature: 37
uniprot:Q8GAT9 [glucosylceramidase(Enzyme) mutant D223G His6-tagged]

H2O + p_Nitrophenyl_beta_D_glucoside -> beta_D_Glucose + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Sodium phosphate
PH: 6.5
Temperature: 37
uniprot:Q8GAT9 [glucosylceramidase(Enzyme) mutant E411A His6-tagged]

H2O + 2,4_Dinitrophenyl_beta_D_glucopyranoside -> beta_D_Glucose + 2,4_Dinitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Sodium phosphate
PH: 6.5
Temperature: 37
uniprot:Q9HCG7 [glucosylceramidase(Enzyme) wildtype isoenzyme Gba2]

H2O + 4_Methylumbelliferyl_beta_D_glucoside -> 4_Methylumbelliferone + beta_D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M Sodium acetate, 10 mM MnCl2, 0.1 mM EDTA, 1 mM Dithioerythritol
PH: 5
Temperature: 37
uniprot:Q9HCG7 [glucosylceramidase(Enzyme) wildtype isoenzyme Gba2]

H2O + 3_beta_D_Glucosido_chenodeoxycholic_acid -> beta_D_Glucose + Chenodeoxycholate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M Sodium acetate, 10 mM MnCl2, 0.1 mM EDTA, 1 mM Dithioerythritol
PH: 5
Temperature: 37
uniprot:Q9HCG7 [glucosylceramidase(Enzyme) wildtype isoenzyme Gba2]

H2O + 4_Methylumbelliferyl_beta_D_galactoside -> 4_Methylumbelliferone + beta_D_Galactose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M Sodium acetate, 10 mM MnCl2, 0.1 mM EDTA, 1 mM Dithioerythritol
PH: 5
Temperature: 37
uniprot:Q9HCG7 [glucosylceramidase(Enzyme) wildtype isoenzyme Gba2]

3_beta_D_Glucosido_lithocholic_acid + H2O -> beta_D_Glucose + 3alpha_Hydroxy_5beta_cholanate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M Sodium acetate, 10 mM MnCl2, 0.1 mM EDTA, 1 mM Dithioerythritol
PH: 5
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-9840310 Glycosphingolipid catabolism
Reactome:R-MMU-9840310 Glycosphingolipid catabolism
Reactome:R-RNO-9840310 Glycosphingolipid catabolism
Reactome:R-CEL-9840310 Glycosphingolipid catabolism
Reactome:R-DME-9840310 Glycosphingolipid catabolism
Reactome:R-CEL-428157 Sphingolipid metabolism
Reactome:R-DME-1660662 Glycosphingolipid metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-RNO-1660662 Glycosphingolipid metabolism
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-428157 Sphingolipid metabolism
Reactome:R-MMU-1660662 Glycosphingolipid metabolism
Reactome:R-HSA-1660662 Glycosphingolipid metabolism
Reactome:R-MMU-428157 Sphingolipid metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1660662 Glycosphingolipid metabolism
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-428157 Sphingolipid metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-428157 Sphingolipid metabolism
WikiPathways:WP4153 Degradation pathway of sphingolipids, including diseases
PathBank:SMP0000349 Gaucher Disease
PathBank:SMP0000348 Globoid Cell Leukodystrophy
PathBank:SMP0000525 Fabry Disease
PathBank:SMP0087244 Sphingolipid Metabolism
PathBank:SMP0000034 Sphingolipid Metabolism
PathBank:SMP0000347 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0000526 Krabbe Disease