EC: 3.2.1.14

chitinase ((1->4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P00698 [chitinase(Enzyme) wildtype]

Chitin + H2O -> Chitin + N_Acetyl_D_glucosamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M sodium acetate
PH: 6
Temperature: 37
uniprot:H2EQQ0 [chitinase(Enzyme) wildtype]

Chitin + H2O -> N_Acetyl_D_glucosamine + Chitin		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M sodium acetate
PH: 6
Temperature: 37
- [chitinase(Enzyme) wildtype]

Chitobiose + H2O -> 2 N_Acetyl_D_glucosamine		 (( (( (E * kcat) ) * (S ^ 2)) ) / (( (Km + (S ^ 2)) ) + (( (S ^ 3) ) / Ki))) buffer: 50 mM Sodium phosphate
PH: 6
Temperature: 36
- [chitinase(Enzyme) wildtype]

H2O + Chitobiose -> 2 N_Acetyl_D_glucosamine		 (( (( (E * kcat) ) * (S ^ 2)) ) / (( (Km + (S ^ 2)) ) + (( (S ^ 3) ) / Ki))) buffer: 50 mM Sodium phosphate
PH: 6
Temperature: 36
- [chitinase(Enzyme) wildtype]

H2O + Chitobiose -> 2 N_Acetyl_D_glucosamine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Sodium phosphate
PH: 6
Temperature: 36
uniprot:P48827 [chitinase(Enzyme) wildtype CHIT42]

H2O + p_Nitrophenyl_N,N'_diacetylchitobiose -> N,N'_Diacetylchitobiose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 6.36 mM Phosphate/KOH
PH: 6
Temperature: 37
uniprot:B2VQD9 [chitinase(Enzyme) wildtype CHIT37]

p_Nitrophenyl_N,N'_diacetylchitobiose + H2O -> p_Nitrophenol + N,N'_Diacetylchitobiose		 (( (Vmax * A) ) / (Km + A)) buffer: 6.36 mM Phosphate/KOH
PH: 6
Temperature: 37
uniprot:B2VQD9 [chitinase(Enzyme) wildtype CHIT37]

Chitin + H2O -> N_Acetyl_D_glucosamine + Chitin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Phosphate/KOH
PH: 6
Temperature: 37
uniprot:Q12713 [chitinase(Enzyme) wildtype CHIT33]

Chitin + H2O -> N_Acetyl_D_glucosamine + Chitin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Phosphate/KOH
PH: 6
Temperature: 37
uniprot:P48827 [chitinase(Enzyme) wildtype CHIT42]

Chitin + H2O -> Chitin + N_Acetyl_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Phosphate/KOH
PH: 6
Temperature: 37
uniprot:Q873Y0 [chitinase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_beta_D_N,N',N''_triacetylchitotrioside -> N,N',N''_Triacetylchitotriose + 4_Methylumbelliferone		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 200 mM sodium phosphate, 100 mM citric acid, 0.1 mg/ml BSA
PH: 5.5
Temperature: 37
uniprot:P29022 [chitinase(Enzyme) wildtype]

H2O + Tetra_N_acetylchitotetraose -> Acetate + Tri_N_acetylchitotetraose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM potassium phosphate
PH: 4
Temperature: 37
uniprot:P29022 [chitinase(Enzyme) wildtype]

Tetra_N_acetylchitotetraose + H2O -> Tri_N_acetylchitotetraose + Acetate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM potassium phosphate
PH: 6
Temperature: 37
uniprot:P29022 [chitinase(Enzyme) wildtype]

Tetra_N_acetylchitotetraose + H2O -> Tri_N_acetylchitotetraose + Acetate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM potassium phosphate
PH: 6
Temperature: 70
uniprot:P29022 [chitinase(Enzyme) wildtype]

Tetra_N_acetylchitotetraose + H2O -> Acetate + Tri_N_acetylchitotetraose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM potassium phosphate
PH: 6
Temperature: 50

Pathways

pathway id name
BioCyc:ARA_PWY-6902 chitin degradation II (Vibrio)
BioCyc:META_PWY-7822 chitin degradation III (Serratia)
PathBank:SMP0000905 Amino Sugar and Nucleotide Sugar Metabolism I
PathBank:SMP0000906 Amino Sugar and Nucleotide Sugar Metabolism II
PathBank:SMP0000914 Amino Sugar and Nucleotide Sugar Metabolism III
PlantCyc:ARA_PWY-6902 chitin degradation II (Vibrio)
Reactome:R-PFA-189085 Digestion of dietary carbohydrate
Reactome:R-PFA-8935690 Digestion
Reactome:R-PFA-8963743 Digestion and absorption
Reactome:R-MMU-189085 Digestion of dietary carbohydrate
Reactome:R-RNO-189085 Digestion of dietary carbohydrate
Reactome:R-HSA-8935690 Digestion
Reactome:R-SCE-8935690 Digestion
Reactome:R-HSA-189085 Digestion of dietary carbohydrate
Reactome:R-MMU-8963743 Digestion and absorption
Reactome:R-RNO-8963743 Digestion and absorption
Reactome:R-SCE-189085 Digestion of dietary carbohydrate
Reactome:R-HSA-8963743 Digestion and absorption
Reactome:R-MMU-8935690 Digestion
Reactome:R-RNO-8935690 Digestion
Reactome:R-SCE-8963743 Digestion and absorption
PathBank:SMP0120818 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120524 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0000240 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120737 Sialuria or French Type Sialuria
PathBank:SMP0120765 Tay-Sachs Disease
PathBank:SMP0000045 Amino Sugar Metabolism
PathBank:SMP0000216 Sialuria or French Type Sialuria
PathBank:SMP0087281 Amino Sugar Metabolism
PathBank:SMP0120744 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120517 Sialuria or French Type Sialuria
PathBank:SMP0120545 Tay-Sachs Disease
PathBank:SMP0000390 Tay-Sachs Disease
PathBank:SMP0120599 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0000534 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0063580 Amino Sugar Metabolism