EC: 3.1.4.2

glycerophosphocholine phosphodiesterase (sn-glycero-3-phosphocholine glycerophosphohydrolase)

enzyme lambda metabolic reaction experiment
- [Ethylenediaminetetraacetic acid]

sn_Glycero_3_phosphocholine + H2O -> Choline + sn_Glycerol_3_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/sodium hydroxide, 12.5 µM EDTA, 12.5 µM EGTA
PH: 9
Temperature: 37
- [glycerophosphocholine phosphodiesterase(Enzyme) wildtype]

H2O + sn_Glycero_3_phosphocholine -> sn_Glycerol_3_phosphate + Choline		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/sodium hydroxide, 12.5 µM EDTA, 12.5 µM EGTA
PH: 10.5
Temperature: 37
- [glycerophosphocholine phosphodiesterase(Enzyme) wildtype]

H2O + sn_Glycero_3_phosphocholine -> Choline + sn_Glycerol_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.4
Temperature: 37
- [glycerophosphocholine phosphodiesterase(Enzyme) wildtype]

sn_Glycero_3_phosphocholine + H2O -> sn_Glycerol_3_phosphate + Choline		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M glycylglycine, 1.5 mM MgCl2
PH: 8.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7409 phospholipid remodeling (phosphatidylethanolamine, yeast)
BioCyc:YEAST_PWY-7409 phospholipid remodeling (phosphatidylethanolamine)
BioCyc:META_PWY-7367 phosphatidylcholine resynthesis via glycerophosphocholine
BioCyc:YEAST_PWY-6952-1 glycerophosphodiester degradation
BioCyc:YEAST_PWY-7367 phosphatidylcholine resynthesis via glycerophosphocholine
Reactome:R-HSA-6814848 Glycerophospholipid catabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-1483152 Hydrolysis of LPE
Reactome:R-HSA-1483115 Hydrolysis of LPC
Reactome:R-HSA-1483257 Phospholipid metabolism
Reactome:R-HSA-1483255 PI Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1483206 Glycerophospholipid biosynthesis
PathBank:SMP0002425 Lysolipid Incorporation into ER
PathBank:SMP0002515 Lysolipid Incorporation into ER PC(16:1(9Z)/16:1(9Z))
PathBank:SMP0002519 Lysolipid Incorporation into ER PC(18:2(9Z,11Z)/18:2(9Z,11Z))
PathBank:SMP0002514 Lysolipid Incorporation into ER PC(16:0/16:0)
PathBank:SMP0002518 Lysolipid Incorporation into ER PC(18:1(9Z)/18:1(9Z))
PathBank:SMP0002512 Lysolipid Incorporation into ER PC(10:0/10:0)
PathBank:SMP0002516 Lysolipid Incorporation into ER PC(16:1(11Z)/16:1(11Z))
PathBank:SMP0002520 Lysolipid Incorporation into ER PC(20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))
PathBank:SMP0002393 Choline Metabolism
PathBank:SMP0002513 Lysolipid Incorporation into ER PC(14:0/14:0)
PathBank:SMP0002517 Lysolipid Incorporation into ER PC(18:0/18:0)