EC: 3.1.4.12

sphingomyelin phosphodiesterase (sphingomyelin cholinephosphohydrolase)

enzyme lambda metabolic reaction experiment
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

H2O + Sphingomyelin -> N_Acylsphingosine + Choline_phosphate		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

Palmitoyllysophosphatidylcholine + H2O -> Monopalmitin + Choline_phosphate		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

H2O + Palmitoyllysophosphatidylcholine -> Monopalmitin + Choline_phosphate		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

1_O_Hexadecyl_lyso_sn_glycero_3_phosphocholine + H2O -> Choline_phosphate + 1_O_Hexadecyl_sn_glycerol		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

Palmitoyllysophosphatidylcholine + H2O -> Choline_phosphate + Monopalmitin		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

Palmitoyllysophosphatidylcholine + H2O -> Choline_phosphate + Monopalmitin		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

Stearoyllysophosphatidylcholine + H2O -> Choline_phosphate + Monostearin		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

Myristoyllysophosphatidylcholine + H2O -> Monomyristin + Choline_phosphate		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

H2O + Arachidoyllysophosphatidylcholine -> Monoarachidin + Choline_phosphate		 (( (( (kcat * enzyme) ) * substrate) ) / (Km + substrate)) buffer: 4 mM Mg2+, 33 mM TES
PH: 7.5
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype isoenzyme Smpd2/Smpd3]

Sphingomyelin + H2O -> Choline_phosphate + N_Acylsphingosine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM mono[tris(hydroxymethyl)aminomethane]maleate, 2.5 mg/ml Triton X-100, 40 mM MgCl
PH: 7.1
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype isoenzyme Smpd2/Smpd3]

H2O + Sphingomyelin -> N_Acylsphingosine + Choline_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM mono[tris(hydroxymethyl)aminomethane]maleate, 2.5 mg/ml Triton X-100, 40 mM MgCl
PH: 7.1
Temperature: 37
uniprot:Q5XIA6 [sphingomyelin phosphodiesterase(Enzyme) wildtype isoenzyme Smpd1]

H2O + Sphingomyelin -> N_Acylsphingosine + Choline_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium acetate, 2.5 mg/ml Triton X-100
PH: 4.8
Temperature: 37
- [sphingomyelin phosphodiesterase(Enzyme) wildtype]

Sphingomyelin + H2O -> Choline_phosphate + N_Acylsphingosine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM tris(hydroxymethyl)aminomethane-HCl, 2.5 mg/ml Triton X-100, 1 mM EDTA, 10 mM MgCl2
PH: 7.2
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY3DJ-11281 sphingomyelin metabolism
Reactome:R-BTA-9840310 Glycosphingolipid catabolism
Reactome:R-HSA-9840310 Glycosphingolipid catabolism
Reactome:R-MMU-9840310 Glycosphingolipid catabolism
Reactome:R-DRE-9840310 Glycosphingolipid catabolism
Reactome:R-RNO-9840310 Glycosphingolipid catabolism
Reactome:R-CEL-9840310 Glycosphingolipid catabolism
Reactome:R-DME-9840310 Glycosphingolipid catabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-428157 Sphingolipid metabolism
Reactome:R-CEL-428157 Sphingolipid metabolism
Reactome:R-DRE-1660662 Glycosphingolipid metabolism
Reactome:R-DME-1660662 Glycosphingolipid metabolism
Reactome:R-HSA-193681 Ceramide signalling
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-RNO-1660662 Glycosphingolipid metabolism
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-428157 Sphingolipid metabolism
Reactome:R-MMU-1660662 Glycosphingolipid metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-HSA-1660662 Glycosphingolipid metabolism
Reactome:R-MMU-428157 Sphingolipid metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-BTA-1660662 Glycosphingolipid metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1660662 Glycosphingolipid metabolism
Reactome:R-DRE-428157 Sphingolipid metabolism
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-428157 Sphingolipid metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-73887 Death Receptor Signaling
Reactome:R-HSA-193704 p75 NTR receptor-mediated signalling
Reactome:R-RNO-428157 Sphingolipid metabolism
WikiPathways:WP4725 Sphingolipid metabolism overview
WikiPathways:WP5181 Roles of ceramides in development of insulin resistance
PathBank:SMP0120702 Globoid Cell Leukodystrophy
PathBank:SMP0120738 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0120810 Krabbe Disease
PathBank:SMP0000349 Gaucher Disease
PathBank:SMP0120701 Gaucher Disease
PathBank:SMP0120809 Fabry Disease
PathBank:SMP0087337 Sphingolipid Metabolism
PathBank:SMP0000348 Globoid Cell Leukodystrophy
PathBank:SMP0000525 Fabry Disease
PathBank:SMP0000034 Sphingolipid Metabolism
PathBank:SMP0000347 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0000526 Krabbe Disease