uniprot:Q13822 [Enzyme wildtype NPP2]
H2O + P1,P3_Bis(5'_adenosyl)_triphosphate -> AMP + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 25 mM Tris/ 25 mM glycine, 140 mM NaCl, 5 mM KCl, 500 µg/ml bovine serum albumin, 1 mM CaCl2, 1 mM MgCl2
PH: 9
Temperature: 37 |
uniprot:P22413 [Enzyme wildtype NPP1]
H2O + P1,P3_Bis(5'_adenosyl)_triphosphate -> AMP + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Hepes, 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2
PH: 8.5
Temperature: 37 |
uniprot:P97675 [Enzyme wildtype NPP3]
H2O + P1,P3_Bis(5'_adenosyl)_triphosphate -> ADP + AMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Hepes, 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2
PH: 8.5
Temperature: 37 |
uniprot:Q58346 [Ni2+]
Bis_4_nitrophenyl_phosphate + H2O -> 4_Nitrophenyl_phosphate + p_Nitrophenol |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Diethanolamine, 40 mM NaCl
PH: 9.8
Temperature: 70 |
uniprot:Q58346 [Ni2+]
H2O + Bis_4_nitrophenyl_phosphate -> p_Nitrophenol + 4_Nitrophenyl_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Diethanolamine, 40 mM NaCl
PH: 9.8
Temperature: 70 |
uniprot:Q58346 [Mn2+]
4_Nitrophenyl_phosphocholine + H2O -> Choline + 4_Nitrophenyl_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Diethanolamine, 40 mM NaCl
PH: 9.8
Temperature: 70 |
uniprot:Q58346 [Ni2+]
p_Nitrophenylthymidine_phosphate + H2O -> dTMP + p_Nitrophenol |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Diethanolamine, 40 mM NaCl
PH: 9.8
Temperature: 70 |
uniprot:Q58346 [Ni2+]
H2O + 4_Nitrophenyl_phosphocholine -> 4_Nitrophenyl_phosphate + Choline |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Diethanolamine, 40 mM NaCl
PH: 9.8
Temperature: 70 |
uniprot:Q58346 [Ni2+]
H2O + Bis_4_nitrophenyl_phosphate -> 4_Nitrophenyl_phosphate + p_Nitrophenol |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Diethanolamine, 40 mM NaCl
PH: 9.8
Temperature: 70 |
- [phosphodiesterase I(Enzyme) wildtype]
Adenylyl_3',5'_guanosine + H2O -> GMP + Adenosine |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 1 mM MnCl2, 0.5 mM EDTA, 7.5 mM dithiothreitol
PH: 7.9
Temperature: 37 |
- [phosphodiesterase I(Enzyme) wildtype]
H2O + p_Nitrophenylthymidine_phosphate -> p_Nitrophenol + dTMP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 1 mM MnCl2, 0.5 mM EDTA, 7.5 mM dithiothreitol
PH: 7.9
Temperature: 37 |
- [phosphodiesterase I(Enzyme) wildtype]
H2O + Adenylyl_3',5'_adenosine -> Adenosine + AMP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 1 mM MnCl2, 0.5 mM EDTA, 7.5 mM dithiothreitol
PH: 7.9
Temperature: 37 |
- [phosphodiesterase I(Enzyme) wildtype]
H2O + Cytidylyl_3',5'_uracil -> Cytidine + UMP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 1 mM MnCl2, 0.5 mM EDTA, 7.5 mM dithiothreitol
PH: 7.9
Temperature: 37 |
- [phosphodiesterase I(Enzyme) wildtype]
Adenylyl_3',5'_cytidine + H2O -> Adenosine + CMP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 1 mM MnCl2, 0.5 mM EDTA, 7.5 mM dithiothreitol
PH: 7.9
Temperature: 37 |
- [phosphodiesterase I(Enzyme) wildtype]
6 H2O + U(pU)6 -> Uridine + 6 UMP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 1 mM MnCl2, 0.5 mM EDTA, 7.5 mM dithiothreitol
PH: 7.9
Temperature: 37 |
- [phosphodiesterase I(Enzyme) wildtype]
ATP + H2O -> AMP + Diphosphate |
(( (V * S) ) / (Km + S)) |
|
buffer: 100 mM glycine-NaOH, 5 mM MgCl2, 100 µM ZnCl2
PH: 9.6
Temperature: 37 |
- [phosphodiesterase I(Enzyme) wildtype]
ATP + H2O -> Diphosphate + AMP |
(( (V * S) ) / (Km + S)) |
|
buffer: 100 mM glycine-NaOH, 5 mM MgCl2, 100 µM ZnCl2
PH: 9.6
Temperature: 37 |