EC: 3.1.3.5

5'-nucleotidase (5'-ribonucleotide phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q969T7 [5'-nucleotidase(Enzyme) wildtype cNIII-like]

H2O + AMP -> Adenosine + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 50 mM KCl, 50 µM Potassium phosphate
PH: 7.5
Temperature: 37
uniprot:Q969T7 [5'-nucleotidase(Enzyme) wildtype cNIII-like]

GMP + H2O -> Guanosine + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 50 mM KCl, 50 µM Potassium phosphate
PH: 7.5
Temperature: 37
uniprot:Q9W197 [5'-nucleotidase(Enzyme) wildtype CG3362]

H2O + AMP -> Phosphate + Adenosine		 (( (kcat * E) ) / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 50 mM KCl, 50 µM Potassium phosphate
PH: 7.5
Temperature: 25
uniprot:Q9W197 [5'-nucleotidase(Enzyme) wildtype CG3362]

GMP + H2O -> Guanosine + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES-KOH, 5 mM MgCl2, 50 mM KCl, 50 µM Potassium phosphate
PH: 7.5
Temperature: 25
- [5'-nucleotidase(Enzyme) wildtype]

ATP + D_2'_Deoxycarbocyclic_guanosine -> ADP + D_2'_Deoxycarbocyclic_guanosine_5'_phosphate		 (( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Imidazole, 500 mM NaCl, 50 mM MgCl2, 10 mM IMP
PH: 6.5
Temperature: 36
- [5'-nucleotidase(Enzyme) wildtype]

Inosine + Phosphate -> IMP + H2O		 (( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Imidazole, 500 mM NaCl, 50 mM MgCl2
PH: 6.5
Temperature: 36
- [5'-nucleotidase(Enzyme) wildtype]

ATP + L_2'_Deoxycarbocyclic_guanosine -> ADP + L_2'_Deoxycarbocyclic_guanosine_5'_phosphate		 (( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Imidazole, 500 mM NaCl, 50 mM MgCl2, 10 mM IMP
PH: 6.5
Temperature: 36
uniprot:P15309 [5'-nucleotidase(Enzyme) wildtype]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate
PH: 5.5
Temperature: 37
uniprot:P15309 [5'-nucleotidase(Enzyme) wildtype neuraminidase treated]

AMP + H2O -> Adenosine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate
PH: 5.5
Temperature: 37
uniprot:P15309 [5'-nucleotidase(Enzyme) wildtype neuraminidase treated]

H2O + dGDP -> Phosphate + dGMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate
PH: 5.5
Temperature: 37
uniprot:P15309 [5'-nucleotidase(Enzyme) wildtype]

H2O + dGDP -> dGMP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M citrate
PH: 5.5
Temperature: 37
uniprot:P07024 [CoCl2]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 20 mM Tris/HCl, 0.5 mM EDTA, 50 mM KCl
PH: 7.5
Temperature: 25
uniprot:P07024 [CoCl2]

AMP + H2O -> Adenosine + Phosphate		 (( (Vmax * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 20 mM Tris/HCl, 0.5 mM EDTA, 50 mM KCl
PH: 7.5
Temperature: 25
uniprot:P07024 [CoCl2]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 0.5 mM EDTA, 50 mM KCl
PH: 7.5
Temperature: 25
uniprot:P07024 [CoCl2]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 0.5 mM EDTA, 50 mM KCl
PH: 7.5
Temperature: 25
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-4]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-4]

IMP + H2O -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-4]

H2O + dCMP -> 2'_Deoxycytidine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-4]

H2O + GMP -> Phosphate + Guanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-1]

AMP + H2O -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-1]

IMP + H2O -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-1]

H2O + GMP -> Guanosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-3]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-2]

AMP + H2O -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-2]

IMP + H2O -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-3]

H2O + IMP -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-1]

H2O + dCMP -> Phosphate + 2'_Deoxycytidine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-2]

dCMP + H2O -> Phosphate + 2'_Deoxycytidine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-3]

dCMP + H2O -> 2'_Deoxycytidine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-3]

GMP + H2O -> Phosphate + Guanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype isoform 5'-NT-2]

H2O + GMP -> Guanosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl
PH: 7.5
Temperature: 37
- [Mg2+]

H2O + IMP -> Phosphate + Inosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 500 mM NaCl, 0.1% Bovine serum albumin
PH: 6.5
Temperature: 37
- [Mg2+]

GMP + H2O -> Phosphate + Guanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 500 mM NaCl, 0.1% Bovine serum albumin
PH: 6.5
Temperature: 37
- [Mg2+]

dIMP + H2O -> Phosphate + Deoxyinosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 500 mM NaCl, 0.1% Bovine serum albumin
PH: 6.5
Temperature: 37
- [Mg2+]

dGMP + H2O -> Deoxyguanosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 500 mM NaCl, 0.1% Bovine serum albumin
PH: 6.5
Temperature: 37
- [Mg2+]

CMP + H2O -> Phosphate + Cytidine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 500 mM NaCl, 0.1% Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mn2+]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

AMP + H2O -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

IMP + H2O -> Phosphate + Inosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

H2O + dGMP -> Phosphate + Deoxyguanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

H2O + XMP -> Xanthosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

dAMP + H2O -> Phosphate + Deoxyadenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

GMP + H2O -> Phosphate + Guanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

dIMP + H2O -> Deoxyinosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [ATP]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [ATP]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [ADP]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

dTMP + H2O -> Thymidine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

IMP + H2O -> Phosphate + Inosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

UMP + H2O -> Phosphate + Uridine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

H2O + CMP -> Phosphate + Cytidine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [ATP]

H2O + GMP -> Phosphate + Guanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mn2+]

IMP + H2O -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

H2O + AMP -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Co2+]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

IMP + H2O -> Phosphate + Inosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Co2+]

IMP + H2O -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

IMP + H2O -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

H2O + GMP -> Phosphate + Guanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:Q6AYP7 [Mg2+]

H2O + GMP -> Phosphate + Guanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole-HCl, 0.1 % Bovine serum albumin
PH: 6.5
Temperature: 37
uniprot:P52307 [5'-nucleotidase(Enzyme) wildtype]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.10 M Tris-HCl, 0.1 unit/ml adenosine deaminase
PH: 7.5
Temperature: 25
uniprot:P52307 [5'-nucleotidase(Enzyme) wildtype]

ADP + H2O -> Diphosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.10 M Tris-HCl, 0.1 unit/ml adenosine deaminase
PH: 7.5
Temperature: 25
uniprot:P52307 [5'-nucleotidase(Enzyme) wildtype]

H2O + ATP -> Triphosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.10 M Tris-HCl, 0.1 unit/ml adenosine deaminase
PH: 7.5
Temperature: 25
uniprot:P52307 [5'-nucleotidase(Enzyme) wildtype]

H2O + Bis_4_nitrophenyl_phosphate -> p_Nitrophenol + 4_Nitrophenyl_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.10 M Tris-HCl
PH: 7.5
Temperature: 25
uniprot:Q6UC93 [Cu2+]

dTMP + H2O -> Thymidine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M Sodium acetate
PH: 5.2
Temperature: 37
uniprot:Q6UC93 [Cu2+]

H2O + AMP -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M Sodium acetate
PH: 4.6
Temperature: 37
uniprot:Q6UC93 [Cu2+]

H2O + GMP -> Guanosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M Sodium acetate
PH: 4.6
Temperature: 37
uniprot:Q6UC93 [Cu2+]

UMP + H2O -> Phosphate + Uridine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M Sodium acetate
PH: 5.2
Temperature: 37
uniprot:Q6UC93 [Cu2+]

CMP + H2O -> Phosphate + Cytidine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M Sodium acetate
PH: 5.2
Temperature: 37
uniprot:P52307 [5'-nucleotidase(Enzyme) wildtype]

4_Nitrophenyl_phenyl_phosphonate + H2O -> Phenyl_phosphonate + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.10 M Tris-HCl
PH: 7.5
Temperature: 25
- [5'-nucleotidase(Enzyme) wildtype]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

IMP + H2O -> Inosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

GMP + H2O -> Guanosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

dGMP + H2O -> Phosphate + Deoxyguanosine		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

H2O + dUMP -> Phosphate + 2'_Deoxyuridine		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

H2O + dAMP -> Phosphate + Deoxyadenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

XMP + H2O -> Xanthosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

H2O + dCMP -> 2'_Deoxycytidine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

UMP + H2O -> Uridine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

H2O + CMP -> Phosphate + Cytidine		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [5'-nucleotidase(Enzyme) wildtype]

H2O + dIMP -> Deoxyinosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 20 mM MgCl2, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [Mg2+]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 62.5 mM imidazole-HCl, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [Mg2+]

AMP + H2O -> Adenosine + Phosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 62.5 mM imidazole-HCl, 2 mg/ml bovine serum albumin
PH: 7.4
Temperature: 37
- [Mg2+]

H2O + AMP -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

H2O + AMP -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

H2O + AMP -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

AMP + H2O -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

H2O + AMP -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
uniprot:Q98RF4 [Mg2+]

AMP + H2O -> Phosphate + Adenosine		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

H2O + AMP -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
- [Mg2+]

H2O + AMP -> Adenosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Glycine/NaOH
PH: 8.5
Temperature: 37
uniprot:P0A840 [Mn2+]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7
Temperature: 37
uniprot:P0A840 [Mn2+]

H2O + AMP -> Adenosine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7
Temperature: 37
uniprot:P76491 [Co2+]

H2O + dTMP -> Thymidine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 8
Temperature: 37
uniprot:P76491 [Co2+]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 8
Temperature: 37
uniprot:P0A840 [Mn2+]

H2O + dGMP -> Deoxyguanosine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7
Temperature: 37
uniprot:P76491 [Co2+]

H2O + dGMP -> Deoxyguanosine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 8
Temperature: 37
uniprot:P0A840 [Mn2+]

GMP + H2O -> Guanosine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7
Temperature: 37
uniprot:P76491 [Co2+]

dAMP + H2O -> Deoxyadenosine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 8
Temperature: 37
uniprot:P0A8Y1 [Mn2+]

dTMP + H2O -> Thymidine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7.5
Temperature: 37
uniprot:P0A8Y1 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7.5
Temperature: 37
uniprot:P0A8Y1 [Mn2+]

H2O + dUMP -> Phosphate + 2'_Deoxyuridine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7.5
Temperature: 37
uniprot:P76491 [Co2+]

H2O + dUMP -> 2'_Deoxyuridine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 8
Temperature: 37
uniprot:P76491 [Co2+]

dCMP + H2O -> Phosphate + 2'_Deoxycytidine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 8
Temperature: 37
uniprot:P0A8Y1 [Mn2+]

UMP + H2O -> Phosphate + Uridine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 7.5
Temperature: 37
uniprot:P76491 [Co2+]

H2O + dIMP -> Phosphate + Deoxyinosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES-K
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-5695 urate biosynthesis/inosine 5'-phosphate degradation
BioCyc:META_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:META_PWY-5695 inosine 5'-phosphate degradation
BioCyc:MOUSE_URSIN-PWY ureide biosynthesis
BioCyc:MOUSE_PWY-6355 phosphate acquisition II
BioCyc:BSUB_PWY-6608 guanosine nucleotides degradation III
BioCyc:BSUB_URSIN-PWY ureide biosynthesis
BioCyc:ECOL316407_PWY-6608 guanosine nucleotides degradation III
BioCyc:BTHE_PWY-6907 thiamin diphosphate biosynthesis III (Staphylococcus)
BioCyc:AGRO_PWY-6608 guanosine nucleotides degradation III
BioCyc:MOUSE_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:MOUSE_PWY-6348 phosphate acquisition
BioCyc:MOUSE_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:BSUB_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:BTHE_PWY-6348 phosphate acquisition
BioCyc:BTHE_PWY-6357 phosphate utilization in cell wall regeneration
BioCyc:HUMAN_PWY-6608 guanosine nucleotides degradation
BioCyc:HUMAN_SALVADEHYPOX-PWY adenosine nucleotides degradation
BioCyc:META_PWY-6608 guanosine nucleotides degradation III
BioCyc:MOUSE_PWY-5695 urate biosynthesis/inosine 5'-phosphate degradation
BioCyc:BSUB_NAD-BIOSYNTHESIS-II NAD salvage pathway III (to nicotinamide riboside)
BioCyc:HUMAN_PWY-6353 purine nucleotides degradation
BioCyc:META_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:META_NAD-BIOSYNTHESIS-II NAD salvage pathway III (to nicotinamide riboside)
BioCyc:ECO_PWY-6608 guanosine nucleotides degradation III
BioCyc:MOUSE_NAD-BIOSYNTHESIS-II NAD salvage pathway II
BioCyc:BSUB_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:BSUB_PWY-5695 inosine 5'-phosphate degradation
BioCyc:SYNEL_PWY-6348 phosphate acquisition
BioCyc:CAULONA1000_NAD-BIOSYNTHESIS-II NAD salvage pathway II
PathBank:SMP0002103 Adenosine Nucleotides Degradation
Reactome:R-HSA-9660826 Purinergic signaling in leishmaniasis infection
Reactome:R-BTA-9748784 Drug ADME
Reactome:R-RNO-9748784 Drug ADME
Reactome:R-HSA-9664424 Cell recruitment (pro-inflammatory response)
Reactome:R-XTR-9748784 Drug ADME
Reactome:R-MMU-9755088 Ribavirin ADME
Reactome:R-XTR-9755088 Ribavirin ADME
Reactome:R-HSA-9658195 Leishmania infection
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-BTA-9755088 Ribavirin ADME
Reactome:R-DDI-9755088 Ribavirin ADME
Reactome:R-RNO-9755088 Ribavirin ADME
Reactome:R-DDI-9748784 Drug ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-HSA-9755088 Ribavirin ADME
Reactome:R-HSA-9824443 Parasitic Infection Pathways
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-2161541 Abacavir metabolism
Reactome:R-DDI-74259 Purine catabolism
Reactome:R-DDI-2161522 Abacavir ADME
Reactome:R-DME-8953854 Metabolism of RNA
Reactome:R-DME-73621 Pyrimidine catabolism
Reactome:R-GGA-15869 Nucleotide metabolism
Reactome:R-GGA-8956319 Nucleotide catabolism
Reactome:R-GGA-1660598 Metabolism
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-8953854 Metabolism of RNA
Reactome:R-MMU-429914 Deadenylation-dependent mRNA decay
Reactome:R-RNO-429958 mRNA decay by 3' to 5' exoribonuclease
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-2161541 Abacavir metabolism
Reactome:R-XTR-15869 Nucleotide metabolism
Reactome:R-XTR-8956319 Nucleotide catabolism
Reactome:R-CEL-429958 mRNA decay by 3' to 5' exoribonuclease
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DDI-8956319 Nucleotide catabolism
Reactome:R-DME-429914 Deadenylation-dependent mRNA decay
Reactome:R-DME-1430728 Metabolism
Reactome:R-GGA-73621 Pyrimidine catabolism
Reactome:R-GGA-419470 Nucleotide metabolism
Reactome:R-HSA-74259 Purine catabolism
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-2161522 Abacavir ADME
Reactome:R-HSA-429914 Deadenylation-dependent mRNA decay
Reactome:R-MMU-74259 Purine catabolism
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-2161522 Abacavir ADME
Reactome:R-RNO-74259 Purine catabolism
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-XTR-2161522 Abacavir ADME
Reactome:R-BTA-15869 Nucleotide metabolism
Reactome:R-BTA-8956319 Nucleotide catabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-73621 Pyrimidine catabolism
Reactome:R-DDI-2161541 Abacavir metabolism
Reactome:R-DME-429958 mRNA decay by 3' to 5' exoribonuclease
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-DME-8956319 Nucleotide catabolism
Reactome:R-GGA-1430728 Metabolism
Reactome:R-HSA-73621 Pyrimidine catabolism
Reactome:R-HSA-196807 Nicotinate metabolism
Reactome:R-HSA-2161541 Abacavir metabolism
Reactome:R-HSA-8953854 Metabolism of RNA
Reactome:R-HSA-429958 mRNA decay by 3' to 5' exoribonuclease
Reactome:R-MMU-73621 Pyrimidine catabolism
Reactome:R-MMU-196807 Nicotinate metabolism
Reactome:R-MMU-2161541 Abacavir metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-73621 Pyrimidine catabolism
Reactome:R-RNO-196807 Nicotinate metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-74259 Purine catabolism
Reactome:R-XTR-2161541 Abacavir metabolism
Reactome:R-BTA-74259 Purine catabolism
Reactome:R-BTA-2161522 Abacavir ADME
Reactome:R-CEL-8953854 Metabolism of RNA
Reactome:R-CEL-429914 Deadenylation-dependent mRNA decay
Reactome:R-DRE-15869 Nucleotide metabolism
Reactome:R-DRE-8956319 Nucleotide catabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-GGA-419474 Purine metabolism
Reactome:R-GGA-421178 Urate synthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-8956319 Nucleotide catabolism
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-8956319 Nucleotide catabolism
Reactome:R-MMU-429958 mRNA decay by 3' to 5' exoribonuclease
Reactome:R-RNO-8953854 Metabolism of RNA
Reactome:R-RNO-429914 Deadenylation-dependent mRNA decay
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-8956319 Nucleotide catabolism
Reactome:R-RNO-2161522 Abacavir ADME
WikiPathways:WP4584 Biomarkers for pyrimidine metabolism disorders
WikiPathways:WP5078 T cell modulation in pancreatic cancer
WikiPathways:WP4225 Pyrimidine metabolism and related diseases
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0087241 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0000048 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000172 beta-Ureidopropionase Deficiency
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0087239 Purine Metabolism
PathBank:SMP0087247 Pyrimidine Metabolism