EC: 2.7.7.13

mannose-1-phosphate guanylyltransferase (GTP:alpha-D-mannose-1-phosphate guanylyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P07874 [Mg2+]

GTP + D_Mannose_1_phosphate -> Diphosphate + GDP_D_mannose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 2 mM NAD+, GDP-mannose dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P07874 [Mg2+]

GDP_D_mannose + Diphosphate -> D_Mannose_1_phosphate + GTP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM MOPS, 1 mM NADP+, 1 mM ADP, 4 mM D-Glucose, Nucleoside 5'-diphosphate kinase, Hexokinase, Glucose 6-phosphate dehadrogenase
PH: 7.6
Temperature: 25
uniprot:P07874 [Mg2+]

GTP + D_Mannose_1_phosphate -> GDP_D_mannose + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 2 mM NAD+, GDP-mannose dehydrogenase
PH: 7.6
Temperature: 25
uniprot:P26404 [mannose-1-phosphate guanylyltransferase(Enzyme) wildtype]

alpha_D_Mannose_1_phosphate + GTP -> GDP_alpha_D_mannose + Diphosphate		 (( (Vmax / (1 + (Km / B))) ) + (B / Kis)) buffer: 50 mM Tris/HCl, 10 mM MgCl2, 0.15 mM NADH, 5 mM fructose 6-phosphate, 1 µM fructose 2,6-bisphosphate, 0.45 U/ml aldolase, 5 U/ml triosephosphate isomerase, 1.7 U/ml glycerol-3-phosphate dehydrogenase, 1 U/ml PPi-PFK
PH: 7.4
Temperature: 30
uniprot:P26404 [mannose-1-phosphate guanylyltransferase(Enzyme) wildtype]

alpha_D_Mannose_1_phosphate + GTP -> Diphosphate + GDP_alpha_D_mannose		 (( (Vmax / (1 + (Km / A))) ) + (A / Kis)) buffer: 50 mM Tris/HCl, 10 mM MgCl2, 0.15 mM NADH, 5 mM fructose 6-phosphate, 1 µM fructose 2,6-bisphosphate, 0.45 U/ml aldolase, 5 U/ml triosephosphate isomerase, 1.7 U/ml glycerol-3-phosphate dehydrogenase, 1 U/ml PPi-PFK
PH: 7.4
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-5659 GDP-mannose biosynthesis
BioCyc:VCHO_PWY-5659 GDP-mannose biosynthesis
BioCyc:ECOL316407_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:HUMAN_PWY-5659 GDP-mannose biosynthesis
BioCyc:META_PWY-7323 superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis
BioCyc:META_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
BioCyc:ECO_PWY-5659 GDP-mannose biosynthesis
BioCyc:MOUSE_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:META_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECO_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:YEAST_PWY-5659 GDP-mannose biosynthesis
BioCyc:MOUSE_PWY-5659 GDP-mannose biosynthesis
BioCyc:ARA_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
BioCyc:ARA_PWY-5659 GDP-mannose biosynthesis
BioCyc:MOUSE_MANGDPMET-PWY GDP-mannose metabolism
BioCyc:MOUSE_PWY-5657 GDP-mannose biosynthesis I
BioCyc:ECOL316407_PWY-5659 GDP-mannose biosynthesis
PathBank:SMP0000842 Mannose Metabolism
PathBank:SMP0000906 Amino Sugar and Nucleotide Sugar Metabolism II
PathBank:SMP0000967 Colanic Acid Building Blocks Biosynthesis
PlantCyc:ARA_PWY-5659 GDP-mannose biosynthesis
PlantCyc:ARA_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
Reactome:R-CEL-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-CEL-446205 Synthesis of GDP-mannose
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-DRE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DDI-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DDI-446205 Synthesis of GDP-mannose
Reactome:R-DME-597592 Post-translational protein modification
Reactome:R-DME-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SPO-446203 Asparagine N-linked glycosylation
Reactome:R-XTR-446203 Asparagine N-linked glycosylation
Reactome:R-CEL-392499 Metabolism of proteins
Reactome:R-CEL-597592 Post-translational protein modification
Reactome:R-CEL-446203 Asparagine N-linked glycosylation
Reactome:R-DRE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DRE-446205 Synthesis of GDP-mannose
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-446203 Asparagine N-linked glycosylation
Reactome:R-DME-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DME-446205 Synthesis of GDP-mannose
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446205 Synthesis of GDP-mannose
Reactome:R-XTR-597592 Post-translational protein modification
Reactome:R-CEL-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DDI-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DME-392499 Metabolism of proteins
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-SPO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SPO-446205 Synthesis of GDP-mannose
Reactome:R-XTR-392499 Metabolism of proteins
Reactome:R-XTR-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-XTR-446205 Synthesis of GDP-mannose
Reactome:R-DRE-597592 Post-translational protein modification
Reactome:R-DRE-446203 Asparagine N-linked glycosylation
Reactome:R-DME-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-446205 Synthesis of GDP-mannose
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-SPO-597592 Post-translational protein modification
Reactome:R-SPO-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-XTR-446219 Synthesis of substrates in N-glycan biosythesis
WikiPathways:WP4521 Glycosylation and related congenital defects
PathBank:SMP0000064 Fructose and Mannose Degradation
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0087197 Fructose and Mannose Degradation
PathBank:SMP0000725 Fructose Intolerance, Hereditary
PathBank:SMP0012444 Mannose Metabolism
PathBank:SMP0000561 Fructosuria