EC: 2.7.3.2

creatine kinase (ATP:creatine N-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P00563 [creatine kinase(Enzyme) wildtype isoenzyme CKM]

ATP + Creatine -> Phosphocreatine + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM triethanolamine-HCl, 10 mM MgCl2, 100 mM KCl, 1 mM phosphoenolpyruvate, 0.18 mM NADH, 10 U/ml porcine muscle lactate dehydrogenase, 10 U/ml rabbit muscle pyruvate kinase
PH: 8
Temperature: 22
uniprot:P00563 [creatine kinase(Enzyme) wildtype isoenzyme CKM]

ATP + Creatine -> Phosphocreatine + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM triethanolamine-HCl, 10 mM MgCl2, 100 mM KCl, 1 mM phosphoenolpyruvate, 0.18 mM NADH, 10 U/ml porcine muscle lactate dehydrogenase, 10 U/ml rabbit muscle pyruvate kinase
PH: 8
Temperature: 22
- [creatine kinase(Enzyme) wildtype]

ADP + Phosphocreatine -> Creatine + ATP		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Imidazole, 10 mM Mg2+, 20 mM D-Glucose, 2 mM NADP+, 2500 U/l Hexokinase from Saccharomyces cerevisiae, 1500 U/l Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae, 5 mM AMP, 20 mM N-Acetyl cysteine
PH: 6.7
Temperature: 25
- [creatine kinase(Enzyme) wildtype]

ADP + Phosphocreatine -> ATP + Creatine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole, 10 mM Mg2+, 20 mM D-Glucose, 2 mM NADP+, 2500 U/l Hexokinase from Saccharomyces cerevisiae, 1500 U/l Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae, 5 mM AMP, 20 mM N-Acetyl cysteine
PH: 6.7
Temperature: 25
- [creatine kinase(Enzyme) wildtype]

Phosphocreatine + ADP -> Creatine + ATP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Imidazole, 10 mM Mg2+, 20 mM D-Glucose, 2 mM NADP+, 2500 U/l Hexokinase from Saccharomyces cerevisiae, 1500 U/l Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae, 5 mM AMP, 20 mM N-Acetyl cysteine
PH: 6.7
Temperature: 30
- [creatine kinase(Enzyme) wildtype]

ADP + Phosphocreatine -> ATP + Creatine		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Imidazole, 10 mM Mg2+, 20 mM D-Glucose, 2 mM NADP+, 2500 U/l Hexokinase from Saccharomyces cerevisiae, 1500 U/l Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae, 5 mM AMP, 20 mM N-Acetyl cysteine
PH: 6.7
Temperature: 30

Pathways

pathway id name
BioCyc:MOUSE_PWY3DJ-35588 creatine biosynthesis
BioCyc:HUMAN_PWY-6158 creatine-phosphate biosynthesis
BioCyc:META_PWY-6158 creatine-phosphate biosynthesis
BioCyc:MOUSE_PWY3DJ-471 creatine-phosphate energy transfer
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-CFA-1430728 Metabolism
Reactome:R-HSA-71288 Creatine metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-71288 Creatine metabolism
Reactome:R-RNO-71288 Creatine metabolism
Reactome:R-BTA-71288 Creatine metabolism
Reactome:R-CFA-71291 Amino acid and derivative metabolism
Reactome:R-GGA-71291 Amino acid and derivative metabolism
Reactome:R-GGA-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CFA-71288 Creatine metabolism
Reactome:R-GGA-71288 Creatine metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4584 Biomarkers for pyrimidine metabolism disorders
WikiPathways:WP497 Urea cycle and metabolism of amino groups
WikiPathways:WP5190 Creatine pathway
WikiPathways:WP5242 Comprehensive IL-17A signaling
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0087178 Arginine and Proline Metabolism