EC: 2.5.1.87

ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] ((2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10–55 isopentenyl units))

enzyme lambda metabolic reaction experiment
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> Diphosphate + poly_cis_Polyprenyl_diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> poly_cis_Polyprenyl_diphosphate + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> poly_cis_Polyprenyl_diphosphate + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> Diphosphate + poly_cis_Polyprenyl_diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> Diphosphate + poly_cis_Polyprenyl_diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> poly_cis_Polyprenyl_diphosphate + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> Diphosphate + poly_cis_Polyprenyl_diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

trans,trans_Farnesyl_diphosphate + Isopentenyl_diphosphate -> Diphosphate + poly_cis_Polyprenyl_diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

trans,trans_Farnesyl_diphosphate + Isopentenyl_diphosphate -> poly_cis_Polyprenyl_diphosphate + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37
- [Mg2+]

Isopentenyl_diphosphate + trans,trans_Farnesyl_diphosphate -> poly_cis_Polyprenyl_diphosphate + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM 2-mercaptoethanol, 1 mg/ml BSA, 10 mM KF, 1% phosphatidylinositol
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6129 dolichol and dolichyl phosphate biosynthesis
BioCyc:META_PWY-6383 mono-trans, poly-cis decaprenyl phosphate biosynthesis
BioCyc:MTBH37RV_PWY-6383 mono-trans, poly-cis decaprenyl phosphate biosynthesis
BioCyc:MTBH37RV_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:MTBCDC1551_PWY-6383 mono-trans, poly-cis decaprenyl phosphate biosynthesis
BioCyc:META_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:MTBCDC1551_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:META_PWY-6129 dolichol and dolichyl phosphate biosynthesis
BioCyc:YEAST_PWY-6129 dolichol and dolichyl phosphate biosynthesis
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-DRE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-446199 Synthesis of dolichyl-phosphate
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-SCE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SPO-446203 Asparagine N-linked glycosylation
Reactome:R-DRE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-3781865 Diseases of glycosylation
Reactome:R-HSA-4755609 Defective DHDDS causes RP59
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SPO-446199 Synthesis of dolichyl-phosphate
Reactome:R-DRE-446199 Synthesis of dolichyl-phosphate
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-446199 Synthesis of dolichyl-phosphate
Reactome:R-SCE-446199 Synthesis of dolichyl-phosphate
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-SPO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DRE-597592 Post-translational protein modification
Reactome:R-DRE-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-5609975 Diseases associated with glycosylation precursor biosynthesis
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SCE-446203 Asparagine N-linked glycosylation
Reactome:R-SPO-597592 Post-translational protein modification
Reactome:R-SPO-446219 Synthesis of substrates in N-glycan biosythesis
PathBank:SMP0002384 Terpenoid Backbone Biosynthesis