EC: 2.4.2.10

orotate phosphoribosyltransferase (orotidine-5'-phosphate:diphosphate phospho-alpha-D-ribosyl-transferase)

enzyme lambda metabolic reaction experiment
- [Mg2+]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris-HCl
PH: 7.2
Temperature: 36
- [Mg2+]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Tris-HCl
PH: 7.2
Temperature: 36
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103Q]

Diphosphate + Orotidine_5'_phosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73A]

Orotidine_5'_phosphate + Diphosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26A]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73Q]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A]

Diphosphate + Orotidine_5'_phosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K19Q]

Orotidine_5'_phosphate + Diphosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K100A]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26A]

Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Uridine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotate_6'_methylester + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Orotidine_6'_methylester_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A]

Orotate_6'_methylester + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_6'_methylester_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate_6'_methylester -> Orotidine_6'_methylester_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103Q]

Orotidine_5'_phosphate + Imidodiphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_imidodiphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotidine_5'_phosphate + Imidodiphosphate -> 5_Phospho_alpha_D_ribose_1_imidodiphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73Q]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Orotidine_5'_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/D125N]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73A]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73A]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73Q]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/D125N]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103Q]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73A]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73Q]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K19Q]

Diphosphate + Orotidine_5'_phosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26A]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

Orotidine_5'_phosphate + Diphosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K100A]

Orotidine_5'_phosphate + Diphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotidine_5'_phosphate + Diphosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotidine_5'_phosphate + Diphosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103Q]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K100A]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73A]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K100A]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73A]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103Q]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73Q]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73A]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Diphosphate + Orotidine_5'_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/D125N]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Diphosphate + Orotidine_5'_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Orotidine_5'_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73A]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/K73Q]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K103A/D125N]

Diphosphate + Orotidine_5'_phosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Orotate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Diphosphate + Orotidine_5'_phosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM Tris HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73Q]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K19Q]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26A]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26A]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K73Q]

5_Phospho_alpha_D_ribose_1_diphosphate + Orotate -> Diphosphate + Orotidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K19Q]

Orotate + 5_Phospho_alpha_D_ribose_1_diphosphate -> Orotidine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Uracil -> Uridine_5'_phosphate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

Uracil + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + Uridine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 75 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

Phosphonoacetate + Orotidine_5'_phosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_phosphonoacetate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K100A]

Phosphonoacetate + Orotidine_5'_phosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_phosphonoacetate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) wildtype]

Orotidine_5'_phosphate + Phosphonoacetate -> 5_Phospho_alpha_D_ribose_1_phosphonoacetate + Orotate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K26Q]

Imidodiphosphate + Orotidine_5'_phosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_imidodiphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30
uniprot:P08870 [orotate phosphoribosyltransferase(Enzyme) mutant K100A]

Orotidine_5'_phosphate + Imidodiphosphate -> Orotate + 5_Phospho_alpha_D_ribose_1_imidodiphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 3 mM MgCl2
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY-5686 UMP biosynthesis
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MOUSE_PWY0-162 pyrimidine ribonucleotides de novo biosynthesis
BioCyc:BSUB_PWY-7791 UMP biosynthesis III
BioCyc:BTHE_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-5686 UMP biosynthesis
BioCyc:CORYNE_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:META_PWY-5686 UMP biosynthesis I
BioCyc:ECO_PWY-5686 UMP biosynthesis I
BioCyc:ARA_PWY-5686 UMP biosynthesis I
BioCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY-5686 UMP biosynthesis I
BioCyc:MTBH37RV_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY-5686 UMP biosynthesis I
BioCyc:ECOO157_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY-5686-1 UMP biosynthesis
BioCyc:ECOL316407_PWY-5686 UMP biosynthesis
BioCyc:PDIF272563_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:CORYNE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7790 UMP biosynthesis II
BioCyc:META_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ECO_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-7790 UMP biosynthesis II
BioCyc:ARA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-5686 UMP biosynthesis
BioCyc:CAULO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:MOUSE_PWY-5686 uridine-5'-phosphate biosynthesis
BioCyc:BSUB_PWY-5686-1 uridine-5'-phosphate biosynthesis
BioCyc:ECOL316407_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:CAULONA1000_PWY0-162 pyrimidine ribonucleotides de novo biosynthesis
BioCyc:CAULONA1000_PWY-5686 uridine-5'-phosphate biosynthesis
BioCyc:BTHE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7791 UMP biosynthesis III
BioCyc:ECO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:YEAST_PYRIMID-RNTSYN-PWY de novo biosynthesis of pyrimidine ribonucleotides
BioCyc:ECOO157_PWY-5686 UMP biosynthesis
BioCyc:CAULO_PWY-5686 UMP biosynthesis
BioCyc:ANTHRA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:PDIF272563_PWY-5686 UMP biosynthesis I
BioCyc:PDIF272563_PWY-5686-1 UMP biosynthesis II
BioCyc:CORYNE_PWY-5686 UMP biosynthesis
PathBank:SMP0000959 Pyrimidine Metabolism
PlantCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY-5686 UMP biosynthesis I
PlantCyc:PLANT_PWY-5686 UMP biosynthesis I
PlantCyc:PLANT_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
Reactome:R-CEL-500753 Pyrimidine biosynthesis
Reactome:R-DDI-8956320 Nucleotide biosynthesis
Reactome:R-DME-500753 Pyrimidine biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-8956320 Nucleotide biosynthesis
Reactome:R-MMU-8956320 Nucleotide biosynthesis
Reactome:R-CEL-15869 Nucleotide metabolism
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-HSA-500753 Pyrimidine biosynthesis
Reactome:R-MMU-500753 Pyrimidine biosynthesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-8956320 Nucleotide biosynthesis
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-500753 Pyrimidine biosynthesis
Reactome:R-DME-8956320 Nucleotide biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
PathBank:SMP0121324 Pyrimidine Metabolism
PathBank:SMP0002370 Pyrimidine Metabolism