EC: 2.3.1.87

aralkylamine N-acetyltransferase (acetyl-CoA:2-arylethylamine N-acetyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q6Z1Y6 [aralkylamine N-acetyltransferase(Enzyme) wildtype delta1-34 OsSNAT2, C-terminal His-tagged]

Serotonin + Acetyl_CoA -> Coenzyme_A + N_Acetylserotonin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 8.8
Temperature: 45
uniprot:Q5KQI6 [aralkylamine N-acetyltransferase(Enzyme) wildtype delta1-83 OsSNAT1, His-tagged]

Serotonin + Acetyl_CoA -> Coenzyme_A + N_Acetylserotonin		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate
PH: 8.8
Temperature: 55
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Serotonin + Acetyl_CoA -> Coenzyme_A + N_Acetylserotonin		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + Serotonin -> Coenzyme_A + N_Acetylserotonin		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Serotonin + Acetyl_CoA -> N_Acetylserotonin + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

2_Phenylethylamine + Acetyl_CoA -> Coenzyme_A + N_Acetylphenylethylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + 2_Phenylethylamine -> Coenzyme_A + N_Acetylphenylethylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

2_Phenylethylamine + Acetyl_CoA -> Coenzyme_A + N_Acetylphenylethylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + Tyramine -> Coenzyme_A + N_Acetyltyramine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + Tryptamine -> N_Acetyltryptamine + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Tyramine + Acetyl_CoA -> N_Acetyltyramine + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q16613 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Tyramine + Acetyl_CoA -> N_Acetyltyramine + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M sodium phosphate, 1 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [Indole-3-ethanol]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (Vmax * A) ) / (Km + (A * (1 + (I / Kii))))) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype GSH-AANAT]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype GSH-AANAT]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (Vmax * A) ) / (Km + (A * (1 + (I / Kii))))) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype GSH-AANAT]

Acetyl_CoA + Tryptamine -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype AANAT]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

Coenzyme_A + N_Bromoacetyltryptamine -> CoA_S_acetyl_tryptamine + Hydrobromic_acid		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 500 mM NaCl, 1 mM EDTA, 50 µg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype]

N_Bromoacetyltryptamine + Coenzyme_A -> Hydrobromic_acid + CoA_S_acetyl_tryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 500 mM NaCl, 1 mM EDTA, 50 µg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q5KQI6 [aralkylamine N-acetyltransferase(Enzyme) mutant 83 N-terminal residues deletion]

Serotonin + Acetyl_CoA -> Coenzyme_A + N_Acetylserotonin		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 8.8
Temperature: 30
uniprot:Q5KQI6 [aralkylamine N-acetyltransferase(Enzyme) mutant 83 N-terminal residues deletion]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 8.8
Temperature: 30
uniprot:Q5KQI6 [aralkylamine N-acetyltransferase(Enzyme) mutant 83 N-terminal residues deletion]

5_Methoxytryptamine + Acetyl_CoA -> Melatonin + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 8.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype GSH-AANAT]

Serotonin + Acetyl_CoA -> N_Acetylserotonin + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [desulfo-CoA]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [desulfo-CoA]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (( (Vmax * A) ) / Km) ) * (1 + (I / Kis))) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [Indole-3-ethanol]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (( (Vmax * A) ) / Km) ) * (1 + (I / Kis))) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype GSH-AANAT]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (A * Kb)) ) + (B * Ka)) ) + (A * B))) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype AANAT]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) wildtype GSH-AANAT]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant Y168F GST-fusion]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120Q GST-fusion]

Acetyl_CoA + Tryptamine -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant Y168F GST-fusion]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H122Q GST-fusion]

Acetyl_CoA + Tryptamine -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120Q/H122Q GST-fusion]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120A GST-fusion]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H122A GST-fusion]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H122Q GST-fusion]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H122A GST-fusion]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120Q/H122Q GST-fusion]

Acetyl_CoA + Tryptamine -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120A GST-fusion]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120Q GST-fusion]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant Y168F GST-fusion]

Coenzyme_A + N_Bromoacetyltryptamine -> Hydrobromic_acid + CoA_S_acetyl_tryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant Y168F GST-fusion]

N_Bromoacetyltryptamine + Coenzyme_A -> CoA_S_acetyl_tryptamine + Hydrobromic_acid		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant Y168F GST-fusion]

alpha_Methyltryptamine + Acetyl_CoA -> N_Acetyl_alpha_methyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120A/H122A GST-fusion]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H122E GST-fusion]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H122E GST-fusion]

Acetyl_CoA + Tryptamine -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120A/H122A GST-fusion]

Tryptamine + Acetyl_CoA -> Coenzyme_A + N_Acetyltryptamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30
uniprot:Q29495 [aralkylamine N-acetyltransferase(Enzyme) mutant H120E GST-fusion]

Tryptamine + Acetyl_CoA -> N_Acetyltryptamine + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M sodium phosphate, 500 mM NaCl, 2 mM EDTA, 0.05 mg/ml bovine serum albumin
PH: 6.8
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:MOUSE_PWY3DJ-5 biosynthesis of serotonin and melatonin
BioCyc:HUMAN_PWY-6030 serotonin and melatonin biosynthesis
BioCyc:META_PWY-6030 serotonin and melatonin biosynthesis
BioCyc:MOUSE_PWY-6030 serotonin and melatonin biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-GGA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-209776 Metabolism of amine-derived hormones
Reactome:R-HSA-209931 Serotonin and melatonin biosynthesis
Reactome:R-MMU-209776 Metabolism of amine-derived hormones
Reactome:R-MMU-209931 Serotonin and melatonin biosynthesis
Reactome:R-RNO-209776 Metabolism of amine-derived hormones
Reactome:R-RNO-209931 Serotonin and melatonin biosynthesis
Reactome:R-GGA-1430728 Metabolism
Reactome:R-GGA-209776 Metabolism of amine-derived hormones
Reactome:R-GGA-209931 Serotonin and melatonin biosynthesis
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4156 Biosynthesis and regeneration of tetrahydrobiopterin and catabolism of phenylalanine
PathBank:SMP0087237 Tryptophan Metabolism
PathBank:SMP0000063 Tryptophan Metabolism