EC: 2.3.1.65

bile acid-CoA:amino acid N-acyltransferase (choloyl-CoA:glycine N-choloyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Taurine + Choloyl_CoA -> Coenzyme_A + Taurocholate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 8.4
Temperature: 37
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Glycine + Choloyl_CoA -> Glycocholate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 8.4
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Taurine + Choloyl_CoA -> Taurocholate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Glycine -> Coenzyme_A + Glycocholate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Glycine -> Coenzyme_A + Glycocholate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Taurine -> Coenzyme_A + Taurocholate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7
Temperature: 37
- [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Taurine + Choloyl_CoA -> Taurocholate + Coenzyme_A		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 100 mM Potassium phosphate
PH: 8
Temperature: 30
- [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Taurine + Choloyl_CoA -> Coenzyme_A + Taurocholate		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 100 mM Potassium phosphate
PH: 8
Temperature: 30
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Taurine -> Taurocholate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 8.4
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Glycine -> Glycocholate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 8.4
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

2_Fluoro_3_aminopropanoate + Choloyl_CoA -> 3alpha,7alpha,12alpha_trihydroxy_5beta_cholan_24_oyl_2_fluoro_3_aminopropanoate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 8.4
Temperature: 37
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Glycine + Lithocholoyl_CoA -> Coenzyme_A + Glycolithocholate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Lithocholoyl_CoA + Taurine -> Coenzyme_A + Lithocholyltaurine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Glycine + Deoxycholoyl_CoA -> Glycodeoxycholate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Deoxycholoyl_CoA + Taurine -> Deoxycholoyltaurine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Deoxycholoyl_CoA + Taurine -> Deoxycholoyltaurine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Deoxycholoyl_CoA + beta_Alanine -> Coenzyme_A + Deoxycholoyl_3_aminopropanoic_acid		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Aminomethanesulfonate + Deoxycholoyl_CoA -> Coenzyme_A + Deoxycholoyl_aminomethanesulfonate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Taurine + Choloyl_CoA -> Coenzyme_A + Taurocholate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Glycine + Choloyl_CoA -> Glycocholate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Chenodeoxycholoyl_CoA + Taurine -> Coenzyme_A + Taurochenodeoxycholate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Glycine + Chenodeoxycholoyl_CoA -> Glycochenodeoxycholate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q63276 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Deoxycholoyl_CoA + Glycine -> Coenzyme_A + Glycodeoxycholate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM sodium phosphate
PH: 7.2
Temperature: 30
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Glycine -> Coenzyme_A + Glycocholate		 (( (Vmax * A) ) / (Ka + A)) buffer: 100 mM K2HPO4
PH: 8.25
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) mutant C235S]

Glycine + Choloyl_CoA -> Coenzyme_A + Glycocholate		 (( (Vmax * A) ) / (Ka + A)) buffer: 100 mM K2HPO4
PH: 8.25
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Taurine -> Taurocholate + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Choloyl_CoA + Taurine -> Taurocholate + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Glycine + Choloyl_CoA -> Coenzyme_A + Glycocholate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Glycine + Choloyl_CoA -> Glycocholate + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Taurine + Chenodeoxycholoyl_CoA -> Coenzyme_A + Taurochenodeoxycholate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Taurine + Chenodeoxycholoyl_CoA -> Coenzyme_A + Taurochenodeoxycholate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Chenodeoxycholoyl_CoA + Glycine -> Coenzyme_A + Glycochenodeoxycholate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37
uniprot:Q14032 [bile acid-CoA:amino acid N-acyltransferase(Enzyme) wildtype]

Chenodeoxycholoyl_CoA + Glycine -> Coenzyme_A + Glycochenodeoxycholate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM potassium phosphate
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY-6061 bile acid biosynthesis, neutral pathway
Reactome:R-HSA-194068 Bile acid and bile salt metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-RNO-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-RNO-192105 Synthesis of bile acids and bile salts
Reactome:R-RNO-159418 Recycling of bile acids and salts
Reactome:R-HSA-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-159418 Recycling of bile acids and salts
Reactome:R-MMU-194068 Bile acid and bile salt metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-MMU-192105 Synthesis of bile acids and bile salts
Reactome:R-MMU-159418 Recycling of bile acids and salts
Reactome:R-RNO-194068 Bile acid and bile salt metabolism
WikiPathways:WP5176 Disorders of bile acid synthesis and biliary transport
PathBank:SMP0120468 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0000318 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0120463 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120467 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0120495 Zellweger Syndrome
PathBank:SMP0000035 Bile Acid Biosynthesis
PathBank:SMP0000314 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0000315 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120652 27-Hydroxylase Deficiency
PathBank:SMP0120477 Familial Hypercholanemia (FHCA)
PathBank:SMP0000317 Familial Hypercholanemia (FHCA)
PathBank:SMP0000316 Zellweger Syndrome
PathBank:SMP0000720 27-Hydroxylase Deficiency
PathBank:SMP0063601 Bile Acid Biosynthesis