EC: 2.1.1.319

type I protein arginine methyltransferase (S-adenosyl-L-methionine:[protein]-L-arginine N-methyltransferase ([protein]-Nomega,Nomega-dimethyl-L-arginine-forming))

enzyme lambda metabolic reaction experiment
uniprot:O60678 [type I protein arginine methyltransferase(Enzyme) wildtype GST-PRMT3]

S_Adenosyl_L_methionine + GRGGFGGRGGFRGGRGG_NH2 -> S_Adenosyl_L_homocysteine + GRGGFGGRGGFRGGRGG_methyl_arginine		 (( (Vmax * A) ) / (A + (Km * (1 + (P / Ki))))) buffer: 10 mM Sodium phosphate
PH: 7.4
Temperature: 30
uniprot:Q9NR22 [type I protein arginine methyltransferase(Enzyme) wildtype GST-PRMT8]

S_Adenosyl_L_methionine + GRGGFGGRGGFRGGRGG_NH2 -> GRGGFGGRGGFRGGRGG_methyl_arginine + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris
PH: 8
Temperature: 30
uniprot:Q99873 [type I protein arginine methyltransferase(Enzyme) wildtype GST-PRMT1]

S_Adenosyl_L_methionine + GRGGFGGRGGFRGGRGG_NH2 -> GRGGFGGRGGFRGGRGG_methyl_arginine + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (A + (Km * (1 + (P / Ki))))) buffer: 10 mM Sodium phosphate
PH: 7.4
Temperature: 30
uniprot:O60678 [type I protein arginine methyltransferase(Enzyme) wildtype GST-PRMT3]

GRGGFGGRGGFRGGRGG_NH2 + S_Adenosyl_L_methionine -> S_Adenosyl_L_homocysteine + GRGGFGGRGGFRGGRGG_methyl_arginine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Sodium phosphate
PH: 7.4
Temperature: 30
uniprot:Q99873 [type I protein arginine methyltransferase(Enzyme) wildtype GST-PRMT1]

S_Adenosyl_L_methionine + GRGGFGGRGGFRGGRGG_NH2 -> GRGGFGGRGGFRGGRGG_methyl_arginine + S_Adenosyl_L_homocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 10 mM Sodium phosphate
PH: 7.4
Temperature: 30
uniprot:P55345 [type I protein arginine methyltransferase(Enzyme) wildtype PRMT2 GST-tagged]

Histone_L_arginine + 2 S_Adenosyl_L_methionine -> Histone_N(omega),N(omega)_dimethyl_L_arginine + 2 S_Adenosyl_L_homocysteine		 (( (( (Vmax * A) ) * B) ) / (( (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (Km_A * B)) ) + (A * B))) buffer: 50 mM Hepes, 10 mM NaCl, 1 mM DTT
PH: 8
Temperature: 37
uniprot:Q63009 [type I protein arginine methyltransferase(Enzyme) wildtype PRMT1 GST-tagged]

Histone_L_arginine + 2 S_Adenosyl_L_methionine -> Histone_N(omega),N(omega)_dimethyl_L_arginine + 2 S_Adenosyl_L_homocysteine		 (( (( (Vmax * A) ) * B) ) / (( (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (Km_A * B)) ) + (A * B))) buffer: 50 mM Hepes, 10 mM NaCl, 1 mM DTT
PH: 8
Temperature: 37
uniprot:Q63009 [type I protein arginine methyltransferase(Enzyme) wildtype PRMT1 GST-tagged]

2 S_Adenosyl_L_methionine + Histone_L_arginine -> Histone_N(omega),N(omega)_dimethyl_L_arginine + 2 S_Adenosyl_L_homocysteine		 (( (( (Vmax * A) ) * B) ) / (( (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (Km_A * B)) ) + (A * B))) buffer: 50 mM Hepes, 10 mM NaCl, 1 mM DTT
PH: 8
Temperature: 37
uniprot:P55345 [type I protein arginine methyltransferase(Enzyme) wildtype PRMT2 GST-tagged]

2 S_Adenosyl_L_methionine + Histone_L_arginine -> Histone_N(omega),N(omega)_dimethyl_L_arginine + 2 S_Adenosyl_L_homocysteine		 (( (( (Vmax * A) ) * B) ) / (( (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (Km_A * B)) ) + (A * B))) buffer: 50 mM Hepes, 10 mM NaCl, 1 mM DTT
PH: 8
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9694516 SARS-CoV-2 Infection
Reactome:R-HSA-9694635 Translation of Structural Proteins
Reactome:R-HSA-9821002 Chromatin modifications during the maternal to zygotic transition (MZT)
Reactome:R-HSA-9821993 Replacement of protamines by nucleosomes in the male pronucleus
Reactome:R-HSA-9694631 Maturation of nucleoprotein
Reactome:R-HSA-9772573 Late SARS-CoV-2 Infection Events
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9816359 Maternal to zygotic transition (MZT)
Reactome:R-XTR-9009391 Extra-nuclear estrogen signaling
Reactome:R-RNO-9009391 Extra-nuclear estrogen signaling
Reactome:R-HSA-9009391 Extra-nuclear estrogen signaling
Reactome:R-MMU-9009391 Extra-nuclear estrogen signaling
Reactome:R-BTA-74160 Gene expression (Transcription)
Reactome:R-BTA-73857 RNA Polymerase II Transcription
Reactome:R-CEL-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
Reactome:R-DRE-3247509 Chromatin modifying enzymes
Reactome:R-DDI-3247509 Chromatin modifying enzymes
Reactome:R-DDI-3214858 RMTs methylate histone arginines
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DME-9006931 Signaling by Nuclear Receptors
Reactome:R-DME-9018519 Estrogen-dependent gene expression
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-HSA-6791312 TP53 Regulates Transcription of Cell Cycle Genes
Reactome:R-MMU-3214858 RMTs methylate histone arginines
Reactome:R-MMU-74160 Gene expression (Transcription)
Reactome:R-MMU-73857 RNA Polymerase II Transcription
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-8939211 ESR-mediated signaling
Reactome:R-MMU-8876725 Protein methylation
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-8878171 Transcriptional regulation by RUNX1
Reactome:R-RNO-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
Reactome:R-RNO-162582 Signaling Pathways
Reactome:R-RNO-9006931 Signaling by Nuclear Receptors
Reactome:R-RNO-9018519 Estrogen-dependent gene expression
Reactome:R-SCE-4839726 Chromatin organization
Reactome:R-SCE-9006931 Signaling by Nuclear Receptors
Reactome:R-SCE-9018519 Estrogen-dependent gene expression
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-SCE-8876725 Protein methylation
Reactome:R-SPO-3247509 Chromatin modifying enzymes
Reactome:R-XTR-4839726 Chromatin organization
Reactome:R-BTA-3214858 RMTs methylate histone arginines
Reactome:R-CEL-9006931 Signaling by Nuclear Receptors
Reactome:R-CEL-9018519 Estrogen-dependent gene expression
Reactome:R-CEL-3247509 Chromatin modifying enzymes
Reactome:R-CEL-3214858 RMTs methylate histone arginines
Reactome:R-CEL-212436 Generic Transcription Pathway
Reactome:R-DRE-162582 Signaling Pathways
Reactome:R-DRE-4839726 Chromatin organization
Reactome:R-DRE-212436 Generic Transcription Pathway
Reactome:R-DDI-4839726 Chromatin organization
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DME-162582 Signaling Pathways
Reactome:R-DME-8939211 ESR-mediated signaling
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-9018519 Estrogen-dependent gene expression
Reactome:R-HSA-8876725 Protein methylation
Reactome:R-HSA-3214858 RMTs methylate histone arginines
Reactome:R-MMU-3247509 Chromatin modifying enzymes
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-8876725 Protein methylation
Reactome:R-RNO-8939211 ESR-mediated signaling
Reactome:R-SCE-162582 Signaling Pathways
Reactome:R-SCE-8939211 ESR-mediated signaling
Reactome:R-SPO-4839726 Chromatin organization
Reactome:R-XTR-8878171 Transcriptional regulation by RUNX1
Reactome:R-XTR-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
Reactome:R-XTR-9006931 Signaling by Nuclear Receptors
Reactome:R-XTR-9018519 Estrogen-dependent gene expression
Reactome:R-BTA-212436 Generic Transcription Pathway
Reactome:R-BTA-4839726 Chromatin organization
Reactome:R-CEL-162582 Signaling Pathways
Reactome:R-CEL-74160 Gene expression (Transcription)
Reactome:R-DRE-8939211 ESR-mediated signaling
Reactome:R-DRE-3214858 RMTs methylate histone arginines
Reactome:R-DRE-8878171 Transcriptional regulation by RUNX1
Reactome:R-DRE-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
Reactome:R-DDI-8939211 ESR-mediated signaling
Reactome:R-HSA-4839726 Chromatin organization
Reactome:R-HSA-212436 Generic Transcription Pathway
Reactome:R-HSA-3700989 Transcriptional Regulation by TP53
Reactome:R-HSA-8878171 Transcriptional regulation by RUNX1
Reactome:R-HSA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
Reactome:R-MMU-212436 Generic Transcription Pathway
Reactome:R-MMU-9006931 Signaling by Nuclear Receptors
Reactome:R-MMU-9018519 Estrogen-dependent gene expression
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-RNO-4839726 Chromatin organization
Reactome:R-RNO-74160 Gene expression (Transcription)
Reactome:R-RNO-73857 RNA Polymerase II Transcription
Reactome:R-SCE-3247509 Chromatin modifying enzymes
Reactome:R-SPO-3214858 RMTs methylate histone arginines
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-XTR-74160 Gene expression (Transcription)
Reactome:R-XTR-73857 RNA Polymerase II Transcription
Reactome:R-XTR-3247509 Chromatin modifying enzymes
Reactome:R-BTA-8878171 Transcriptional regulation by RUNX1
Reactome:R-BTA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
Reactome:R-BTA-3247509 Chromatin modifying enzymes
Reactome:R-CEL-8939211 ESR-mediated signaling
Reactome:R-CEL-4839726 Chromatin organization
Reactome:R-CEL-73857 RNA Polymerase II Transcription
Reactome:R-CEL-8878171 Transcriptional regulation by RUNX1
Reactome:R-DRE-9006931 Signaling by Nuclear Receptors
Reactome:R-DRE-9018519 Estrogen-dependent gene expression
Reactome:R-DRE-74160 Gene expression (Transcription)
Reactome:R-DRE-73857 RNA Polymerase II Transcription
Reactome:R-DDI-8876725 Protein methylation
Reactome:R-DDI-162582 Signaling Pathways
Reactome:R-DDI-9006931 Signaling by Nuclear Receptors
Reactome:R-DDI-9018519 Estrogen-dependent gene expression
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-8939211 ESR-mediated signaling
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-3247509 Chromatin modifying enzymes
Reactome:R-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
Reactome:R-MMU-4839726 Chromatin organization
Reactome:R-MMU-8878171 Transcriptional regulation by RUNX1
Reactome:R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
Reactome:R-RNO-3247509 Chromatin modifying enzymes
Reactome:R-RNO-3214858 RMTs methylate histone arginines
Reactome:R-RNO-212436 Generic Transcription Pathway
Reactome:R-SCE-3214858 RMTs methylate histone arginines
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SPO-597592 Post-translational protein modification
Reactome:R-SPO-8876725 Protein methylation
Reactome:R-XTR-212436 Generic Transcription Pathway
Reactome:R-XTR-162582 Signaling Pathways
Reactome:R-XTR-8939211 ESR-mediated signaling
Reactome:R-XTR-3214858 RMTs methylate histone arginines
WikiPathways:WP5381 Smith-Magenis and Potocki-Lupski syndrome copy number variation
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0108205 BTG Family Proteins and Cell Cycle Regulation
PathBank:SMP0087314 Histidine Metabolism
PathBank:SMP0063773 BTG Family Proteins and Cell Cycle Regulation
PathBank:SMP0120713 Histidinemia
PathBank:SMP0000044 Histidine Metabolism
PathBank:SMP0000191 Histidinemia
PathBank:SMP0087219 Histidine Metabolism
PathBank:SMP0108235 BTG Family Proteins and Cell Cycle Regulation