EC: 1.8.4.12

peptide-methionine (R)-S-oxide reductase (peptide-methionine:thioredoxin-disulfide S-oxidoreductase [methionine (R)-S-oxide-forming])

enzyme lambda metabolic reaction experiment
uniprot:Q9C5C8 [peptide-methionine (R)-S-oxide reductase(Enzyme) wildtype N-terminal His6-tag]

Reduced_thioredoxin + L_Methionine_(R)_S_oxide -> L_Methionine + Oxidized_thioredoxin + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM Tris-HCl, 1 mM EDTA, 200 µM NADPH, 1.3 µM Arabidopsis NADPH Trx reductase
PH: 8
Temperature: 30
uniprot:Q9C5C8 [peptide-methionine (R)-S-oxide reductase(Enzyme) wildtype N-terminal His6-tag]

Reduced_thioredoxin + N_Acetyl_L_methionine_(R)_sulfoxide -> Oxidized_thioredoxin + N_Acetyl_L_methionine + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM Tris-HCl, 1 mM EDTA, 200 µM NADPH, 1.3 µM Arabidopsis NADPH Trx reductase
PH: 8
Temperature: 30
uniprot:Q9C5C8 [peptide-methionine (R)-S-oxide reductase(Enzyme) wildtype N-terminal His6-tag]

Reduced_thioredoxin + L_Methionine_(R)_S_oxide -> Oxidized_thioredoxin + H2O + L_Methionine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM Tris-HCl, 1 mM EDTA, 200 µM NADPH, 1.3 µM Arabidopsis NADPH Trx reductase
PH: 8
Temperature: 30
uniprot:Q9C8M2 [peptide-methionine (R)-S-oxide reductase(Enzyme) wildtype N-terminal His6-tag]

Dabsyl_L_methionine_(R)_sulfoxide + Dithioerythritol -> Dithioerythritol_disulfide + Dabsyl_L_methionine + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 15 mM HEPES, 10 mM MgCl2, 30 mM KCl
PH: 8
Temperature: 37
uniprot:Q9C5C8 [peptide-methionine (R)-S-oxide reductase(Enzyme) wildtype N-terminal His6-tag]

Dithioerythritol + Dabsyl_L_methionine_(R)_sulfoxide -> Dithioerythritol_disulfide + Dabsyl_L_methionine + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 15 mM HEPES, 10 mM MgCl2, 30 mM KCl
PH: 8
Temperature: 37

Pathways

pathway id name
Reactome:R-DME-392499 Metabolism of proteins
Reactome:R-DME-5676934 Protein repair
PathBank:SMP0120718 Hypermethioninemia
PathBank:SMP0000177 Cystathionine beta-Synthase Deficiency
PathBank:SMP0000340 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0087342 Methionine Metabolism
PathBank:SMP0120853 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0120471 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120487 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120519 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120527 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000033 Methionine Metabolism
PathBank:SMP0000222 Glycine N-Methyltransferase Deficiency
PathBank:SMP0063637 Methionine Metabolism
PathBank:SMP0120634 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0000341 Hypermethioninemia
PathBank:SMP0000221 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120691 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120707 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120739 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120747 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0120498 Hypermethioninemia
PathBank:SMP0000214 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000570 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0087483 Methionine Metabolism