uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA F52L]
Reduced_thioredoxin + N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide -> Oxidized_thioredoxin + N_Acetyl_L_methionine_N_methylamide + H2O |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA H186A]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> N_Acetyl_L_methionine_N_methylamide + H2O + Oxidized_thioredoxin |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) wildtype MsrA]
Reduced_thioredoxin + N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide -> H2O + N_Acetyl_L_methionine_N_methylamide + Oxidized_thioredoxin |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA W53F]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> Oxidized_thioredoxin + H2O + N_Acetyl_L_methionine_N_methylamide |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA W53A]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> Oxidized_thioredoxin + H2O + N_Acetyl_L_methionine_N_methylamide |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA H186N]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> Oxidized_thioredoxin + H2O + N_Acetyl_L_methionine_N_methylamide |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA Y197A]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> N_Acetyl_L_methionine_N_methylamide + Oxidized_thioredoxin + H2O |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA D129A]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> N_Acetyl_L_methionine_N_methylamide + H2O + Oxidized_thioredoxin |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA D129N]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> H2O + Oxidized_thioredoxin + N_Acetyl_L_methionine_N_methylamide |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA Y189F]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> H2O + N_Acetyl_L_methionine_N_methylamide + Oxidized_thioredoxin |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA Y189A]
Reduced_thioredoxin + N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide -> H2O + N_Acetyl_L_methionine_N_methylamide + Oxidized_thioredoxin |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:Q9JWM8 [peptide-methionine (S)-S-oxide reductase(Enzyme) mutant MsrA Y197F]
N_Acetyl_L_methionine_R,S_sulfoxide_N_methylamide + Reduced_thioredoxin -> N_Acetyl_L_methionine_N_methylamide + Oxidized_thioredoxin + H2O |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 2 mM EDTA, 0.3 mM NADPH, 1.2 µM Thioredoxin reductase from Escherichia coli
PH: 8
Temperature: 25 |
uniprot:P08761 [peptide-methionine (S)-S-oxide reductase(Enzyme) wildtype]
L_Methionine_S_oxide + 1,4_Dithiothreitol -> Oxidized_dithiothreitol + H2O + L_Methionine |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Na2HPO4, 1 mM DTPA
PH: 7.4
Temperature: 25 |
uniprot:P08761 [peptide-methionine (S)-S-oxide reductase(Enzyme) wildtype]
Pro_Met(O)_Ala_Ile_Lys_Lys + 1,4_Dithiothreitol -> Pro_Met_Ala_Ile_Lys_Lys + H2O + Oxidized_dithiothreitol |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Na2HPO4, 1 mM DTPA
PH: 7.4
Temperature: 25 |