EC: 1.6.5.2

NAD(P)H dehydrogenase (quinone) (NAD(P)H:quinone oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P9WHH7 [FAD]

NADH + H+ + 2,6_Dimethyl_1,4_benzoquinone -> NAD+ + 2,6_Dimethylhydroquinone		 (( (( (kcat * E) ) * A) ) / (( (KmA * (1 + (I / Ki))) ) + A)) buffer: 100 mM Hepes, 20 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P9WHH7 [ADPribose 2'-phosphate]

2,6_Dimethyl_1,4_benzoquinone + H+ + NADH -> 2,6_Dimethylhydroquinone + NAD+		 (( (( (kcat * E) ) * A) ) / (( (KmA * (1 + (I / Ki))) ) + A)) buffer: 100 mM Hepes, 20 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P9WHH7 [FAD]

5_Hydroxy_1,4_naphthoquinone + H+ + NADH -> NAD+ + 1,4,5_Naphthalenetriol		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (KmA * B) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM Hepes
PH: 7.5
Temperature: 25
uniprot:P9WHH7 [FAD]

NADPH + 5_Hydroxy_1,4_naphthoquinone + H+ -> 1,4,5_Naphthalenetriol + NADP+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (KmA * B) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM Hepes
PH: 7.5
Temperature: 25
uniprot:P9WHH7 [FAD]

NADPH + H+ + 2,6_Dimethyl_1,4_benzoquinone -> 2,6_Dimethylhydroquinone + NADP+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (KmA * B) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM Hepes
PH: 7.5
Temperature: 25
uniprot:P9WHH7 [FAD]

H+ + 2,6_Dimethyl_1,4_benzoquinone + NADH -> NAD+ + 2,6_Dimethylhydroquinone		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (KmA * B) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM Hepes
PH: 7.5
Temperature: 25
uniprot:P9WHH7 [FAD]

NADPH + thio_NAD+ -> NADP+ + thio_NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes
PH: 7.5
Temperature: 25
uniprot:P9WHH7 [FAD]

thio_NAD+ + NADH -> NAD+ + thio_NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes
PH: 7.5
Temperature: 25
uniprot:Q8NJJ9 [NAD(P)H dehydrogenase (quinone)(Enzyme) wildtype His-tagged]

Hydroquinone + NADPH + H+ + O2 -> NADP+ + H2O + Benzene_1,2,4_triol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate
PH: 6.5
Temperature: 25
uniprot:Q8NJJ9 [NAD(P)H dehydrogenase (quinone)(Enzyme) wildtype His-tagged]

1,4_Benzoquinone + H+ + NADPH -> NADP+ + Hydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate
PH: 6.5
Temperature: 25
uniprot:Q8NJJ9 [NAD(P)H dehydrogenase (quinone)(Enzyme) wildtype His-tagged]

H+ + NADPH + 2,3_Dichloro_5,6_dicyano_1,4_benzoquinone -> NADP+ + 2,3_Dichloro_5,6_dicyanohydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate
PH: 6.5
Temperature: 25
uniprot:Q8NJJ9 [NAD(P)H dehydrogenase (quinone)(Enzyme) wildtype His-tagged]

NADPH + Phenyl_1,4_benzoquinone + H+ -> Phenylhydroquinone + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM sodium phosphate
PH: 6.5
Temperature: 25
uniprot:O29904 [Riboflavin-5-phosphate]

NADH + H+ + 1,4_Benzoquinone -> Hydroquinone + NAD+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM MOPS
PH: 7.2
Temperature: 37
uniprot:O29904 [Riboflavin-5-phosphate]

2 Hexacyanoferrate(III) + NADPH -> NADP+ + 2 Hexacyanoferrate(II) + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM MOPS
PH: 7.2
Temperature: 37
uniprot:P0A8G6 [Riboflavin-5-phosphate]

NADH + H+ + 1,4_Benzoquinone -> Hydroquinone + NAD+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM MOPS
PH: 7.2
Temperature: 37
uniprot:O29904 [Riboflavin-5-phosphate]

NADH + 2 Hexacyanoferrate(III) -> 2 Hexacyanoferrate(II) + H+ + NAD+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM MOPS
PH: 7.2
Temperature: 37
uniprot:P0A8G6 [Riboflavin-5-phosphate]

NADH + 2 Hexacyanoferrate(III) -> NAD+ + H+ + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM MOPS
PH: 7.2
Temperature: 37
uniprot:P0A8G6 [Riboflavin-5-phosphate]

NADPH + 2 Hexacyanoferrate(III) -> H+ + NADP+ + 2 Hexacyanoferrate(II)		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM MOPS
PH: 7.2
Temperature: 37
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant N143A]

Methyl_1,4_benzoquinone + NADPH -> NADP+ + Methylhydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant W139F]

NADPH + Methyl_1,4_benzoquinone -> Methylhydroquinone + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant W139I]

Methyl_1,4_benzoquinone + NADPH -> NADP+ + Methylhydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant W139A]

Methyl_1,4_benzoquinone + NADPH -> NADP+ + Methylhydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant N143L]

Methyl_1,4_benzoquinone + NADPH -> NADP+ + Methylhydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant N143L]

NADPH + Methyl_1,4_benzoquinone -> Methylhydroquinone + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant N143A]

NADPH + Methyl_1,4_benzoquinone -> Methylhydroquinone + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) wildtype]

Methyl_1,4_benzoquinone + NADPH -> NADP+ + Methylhydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) wildtype]

NADPH + Methyl_1,4_benzoquinone -> NADP+ + Methylhydroquinone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36
uniprot:P39315 [NAD(P)H dehydrogenase (quinone)(Enzyme) mutant Y140F]

Methyl_1,4_benzoquinone + NADPH -> Methylhydroquinone + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 36

Pathways

pathway id name
BioCyc:ARA_PWY-3781 aerobic respiration I (cytochrome c)
BioCyc:HUMAN_PWY-7999 vitamin K-epoxide cycle
BioCyc:ARA_PWY-4302 aerobic respiration III (alternative oxidase pathway)
BioCyc:ARA_PWY-5083 NAD/NADH phosphorylation and dephosphorylation
BioCyc:META_PWY-7999 vitamin K-epoxide cycle
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PlantCyc:ARA_PWY-4302 aerobic respiration III (alternative oxidase pathway)
PlantCyc:ARA_PWY-3781 aerobic respiration I (cytochrome c)
PlantCyc:ARA_PWY-5083 NAD/NADH phosphorylation and dephosphorylation
Reactome:R-HSA-9818027 NFE2L2 regulating anti-oxidant/detoxification enzymes
Reactome:R-HSA-9755511 KEAP1-NFE2L2 pathway
Reactome:R-HSA-9711123 Cellular response to chemical stress
Reactome:R-HSA-9759194 Nuclear events mediated by NFE2L2
Reactome:R-HSA-351202 Metabolism of polyamines
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-351202 Metabolism of polyamines
Reactome:R-RNO-351202 Metabolism of polyamines
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-RNO-350562 Regulation of ornithine decarboxylase (ODC)
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8953897 Cellular responses to stimuli
WikiPathways:WP5186 Vitamin K metabolism and activation of dependent proteins
PathBank:SMP0000327 Phenytoin (Antiarrhythmic) Action Pathway
PathBank:SMP0083297 NAD+ Signalling Pathway (Cancer)
PathBank:SMP0084271 NAD+ Signalling and Aging
PathBank:SMP0000650 Doxorubicin Metabolism Pathway
PathBank:SMP0000464 Vitamin K Metabolism