EC: 1.2.3.1

aldehyde oxidase (aldehyde:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

N_(2_Dimethylaminoethyl)acridine_4_carboxamide + O2 + H2O -> N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + N_(2_Dimethylaminoethyl)acridine_4_carboxamide -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

N_(2_Dimethylaminoethyl)acridine_4_carboxamide + O2 + H2O -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + N_(2_Dimethylaminoethyl)acridine_4_carboxamide -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

N_(2_Dimethylaminoethyl)acridine_4_carboxamide + O2 + H2O -> N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + N_(2_Dimethylaminoethyl)acridine_4_carboxamide + H2O -> N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + N_(2_Dimethylaminoethyl)acridine_4_carboxamide -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + N_(2_Dimethylaminoethyl)acridine_4_carboxamide + H2O -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + Benzaldehyde + O2 -> Benzoate + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + Benzaldehyde + O2 -> Benzoate + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + Benzaldehyde -> Benzoate + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + Benzaldehyde + H2O -> H2O2 + Benzoate		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + N_(2_Dimethylaminoethyl)acridine_4_carboxamide + H2O -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

N_(2_Dimethylaminoethyl)acridine_4_carboxamide + O2 + H2O -> N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + N_(2_Dimethylaminoethyl)acridine_4_carboxamide -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + N_(2_Dimethylaminoethyl)acridine_4_carboxamide -> N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + N_(2_Dimethylaminoethyl)acridine_4_carboxamide + O2 -> N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate
PH: 7.8
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + Benzaldehyde -> H2O2 + Benzoate		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

Benzaldehyde + H2O + O2 -> H2O2 + Benzoate		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + Benzaldehyde + O2 -> Benzoate + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

Benzaldehyde + O2 + H2O -> H2O2 + Benzoate		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + Benzaldehyde -> H2O2 + Benzoate		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + Benzaldehyde -> Benzoate + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + Benzaldehyde + O2 -> Benzoate + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + Benzaldehyde + H2O -> Benzoate + H2O2		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + Benzaldehyde -> H2O2 + Benzoate		 (( (V * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

Benzaldehyde + H2O + O2 -> H2O2 + Benzoate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + Benzaldehyde -> H2O2 + Benzoate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.5 mM sodium phosphate, 0.1 mM EDTA, 1 M ammonium chloride
PH: 7.8
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

H2O + Benzaldehyde + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + Benzoate		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

H2O + 2 2,6_Dichloroindophenol + Benzaldehyde -> Benzoate + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

Benzaldehyde + H2O + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + Benzoate		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

2 2,6_Dichloroindophenol + Phthalazine + H2O -> 1(2H)_Phthalazinone + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

2 2,6_Dichloroindophenol + Phthalazine + H2O -> 2 Reduced_2,6_dichlorophenolindophenol + 1(2H)_Phthalazinone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

H2O + 2 2,6_Dichloroindophenol + Chloroquinazolinone -> 2_Chloroquinazolin_4_ol + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

Chloroquinazolinone + H2O + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + 2_Chloroquinazolin_4_ol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

O2 + H2O + Phenanthridine -> H2O2 + Phenanthridone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

Phenanthridine + H2O + O2 -> H2O2 + Phenanthridone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

2 2,6_Dichloroindophenol + Benzaldehyde + H2O -> Benzoate + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

Benzaldehyde + 2 2,6_Dichloroindophenol + H2O -> 2 Reduced_2,6_dichlorophenolindophenol + Benzoate		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

Phthalazine + H2O + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + 1(2H)_Phthalazinone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

H2O + 2 2,6_Dichloroindophenol + Phthalazine -> 1(2H)_Phthalazinone + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

Chloroquinazolinone + H2O + 2 2,6_Dichloroindophenol -> 2_Chloroquinazolin_4_ol + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

2 2,6_Dichloroindophenol + H2O + Chloroquinazolinone -> 2 Reduced_2,6_dichlorophenolindophenol + 2_Chloroquinazolin_4_ol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

2 2,6_Dichloroindophenol + Chloroquinazolinone + H2O -> 2 Reduced_2,6_dichlorophenolindophenol + 2_Chloroquinazolin_4_ol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

H2O + O2 + Phenanthridine -> Phenanthridone + H2O2		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

Phenanthridine + H2O + O2 -> Phenanthridone + H2O2		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

Benzaldehyde + 2 2,6_Dichloroindophenol + H2O -> Benzoate + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

Benzaldehyde + H2O + 2 2,6_Dichloroindophenol -> Benzoate + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

2 2,6_Dichloroindophenol + Phthalazine + H2O -> 2 Reduced_2,6_dichlorophenolindophenol + 1(2H)_Phthalazinone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

H2O + Phthalazine + 2 2,6_Dichloroindophenol -> 1(2H)_Phthalazinone + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R921H His6-tagged]

H2O + Phthalazine + 2 2,6_Dichloroindophenol -> 1(2H)_Phthalazinone + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R921H His6-tagged]

H2O + Chloroquinazolinone + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + 2_Chloroquinazolin_4_ol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R921H His6-tagged]

Chloroquinazolinone + 2 2,6_Dichloroindophenol + H2O -> 2_Chloroquinazolin_4_ol + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

Phenanthridine + O2 + H2O -> Phenanthridone + H2O2		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant N1135S His6-tagged]

H2O + Phenanthridine + O2 -> Phenanthridone + H2O2		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype His6-tagged]

Benzaldehyde + H2O + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + Benzoate		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q9Z0U5 [aldehyde oxidase(Enzyme) wildtype]

Famciclovir + O2 + H2O -> H2O2 + 6_Oxo_famciclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:H9TB17 [aldehyde oxidase(Enzyme) wildtype]

6_Deoxypenciclovir + O2 + H2O -> H2O2 + Penciclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:P80456 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + 6_Deoxypenciclovir -> 8_Oxo_6_deoxypenciclovir + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + Phenanthridine + O2 -> Phenanthridone + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:P80456 [aldehyde oxidase(Enzyme) wildtype]

O2 + Isovanillin + H2O -> H2O2 + Isovanillic_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:Q9Z0U5 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + Phenanthridine -> Phenanthridone + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

O2 + N_(2_Dimethylaminoethyl)acridine_4_carboxamide + H2O -> H2O2 + N_(2_Dimethylaminoethyl)_9_oxo_10H_acridine_4_carboxamide		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM potassium phosphate, 0.1 mM EDTA, 1 % (v/v) DMSO
PH: 7.4
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R921H His6-tagged]

2 2,6_Dichloroindophenol + Benzaldehyde + H2O -> 2 Reduced_2,6_dichlorophenolindophenol + Benzoate		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R921H His6-tagged]

2 2,6_Dichloroindophenol + Benzaldehyde + H2O -> Benzoate + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

Phthalazine + H2O + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + 1(2H)_Phthalazinone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

2 2,6_Dichloroindophenol + Phthalazine + H2O -> 2 Reduced_2,6_dichlorophenolindophenol + 1(2H)_Phthalazinone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R921H His6-tagged]

2 2,6_Dichloroindophenol + Phthalazine + H2O -> 2 Reduced_2,6_dichlorophenolindophenol + 1(2H)_Phthalazinone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

H2O + Chloroquinazolinone + 2 2,6_Dichloroindophenol -> 2 Reduced_2,6_dichlorophenolindophenol + 2_Chloroquinazolin_4_ol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

H2O + 2 2,6_Dichloroindophenol + Chloroquinazolinone -> 2 Reduced_2,6_dichlorophenolindophenol + 2_Chloroquinazolin_4_ol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

H2O + Phenanthridine + O2 -> H2O2 + Phenanthridone		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant R802C His6-tagged]

O2 + H2O + Phenanthridine -> Phenanthridone + H2O2		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) mutant H1297R His6-tagged]

H2O + 2 2,6_Dichloroindophenol + Chloroquinazolinone -> 2_Chloroquinazolin_4_ol + 2 Reduced_2,6_dichlorophenolindophenol		 (( (( (kcat * E) ) * A) ) / (Km_A + A)) buffer: 50 mM Tris, 200 mM NaCl, 1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

6_Deoxypenciclovir + H2O + O2 -> Penciclovir + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + Famciclovir -> H2O2 + 6_Oxo_famciclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:H9TB17 [aldehyde oxidase(Enzyme) wildtype]

6_Deoxypenciclovir + O2 + H2O -> Penciclovir + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:Q9Z0U5 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + 6_Deoxypenciclovir -> Penciclovir + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:P80456 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + 6_Deoxypenciclovir -> H2O2 + Penciclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:H9TB17 [aldehyde oxidase(Enzyme) wildtype]

O2 + H2O + Famciclovir -> H2O2 + 6_Oxo_famciclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:Q9Z0U5 [FAD]

O2 + H2O + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_4_yl]amino]benzonitrile -> 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_2_one_4_yl]amino]benzonitrile + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Phosphate, 1.0 mM K3Fe(CN)6, 0.13 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q9Z0U5 [FAD]

O2 + H2O + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_4_yl]amino]benzonitrile -> H2O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_2_one_4_yl]amino]benzonitrile		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Phosphate, 1.0 mM K3Fe(CN)6, 0.13 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q9Z0U5 [FAD]

O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_4_yl]amino]benzonitrile + H2O -> 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_2_one_4_yl]amino]benzonitrile + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Phosphate, 1.0 mM K3Fe(CN)6, 0.13 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q9Z0U5 [FAD]

H2O + O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_4_yl]amino]benzonitrile -> H2O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_2_one_4_yl]amino]benzonitrile		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Phosphate, 1.0 mM K3Fe(CN)6, 0.13 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q9Z0U5 [FAD]

4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_4_yl]amino]benzonitrile + O2 + H2O -> H2O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_2_one_4_yl]amino]benzonitrile		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Phosphate, 1.0 mM K3Fe(CN)6, 0.13 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q9Z0U5 [FAD]

4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_4_yl]amino]benzonitrile + H2O + O2 -> H2O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[d]pyrimidin_2_one_4_yl]amino]benzonitrile		 (( (Vmax * S) ) / (Km + S)) buffer: 80 mM Phosphate, 1.0 mM K3Fe(CN)6, 0.13 mM EDTA
PH: 7.4
Temperature: 37
uniprot:Q06278 [aldehyde oxidase(Enzyme) wildtype]

Pyridoxal + H2O + O2 -> 4_Pyridoxate + O2._		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Potassium phosphate
PH: 7
Temperature: 37
uniprot:Q5FB27 [aldehyde oxidase(Enzyme) wildtype]

4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile + O2 + H2O -> Oxidized_4_[[(7S)_7_hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M phosphate, 1.0 mM K3Fe(CN)6, 0.13 M EDTA
PH: 6
Temperature: 37
uniprot:Q9Z0U5 [aldehyde oxidase(Enzyme) wildtype]

4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile + O2 + H2O -> H2O2 + Oxidized_4_[[(7S)_7_hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M phosphate, 1.0 mM K3Fe(CN)6, 0.13 M EDTA
PH: 6
Temperature: 37
uniprot:Q5FB27 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile -> H2O2 + Oxidized_4_[[(7S)_7_hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M phosphate, 1.0 mM K3Fe(CN)6, 0.13 M EDTA
PH: 6
Temperature: 37
uniprot:Q9Z0U5 [aldehyde oxidase(Enzyme) wildtype]

4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile + H2O + O2 -> Oxidized_4_[[(7S)_7_hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M phosphate, 1.0 mM K3Fe(CN)6, 0.13 M EDTA
PH: 6
Temperature: 37
uniprot:Q5FB27 [aldehyde oxidase(Enzyme) wildtype]

H2O + O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile -> H2O2 + Oxidized_4_[[(7S)_7_hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M phosphate, 1.0 mM K3Fe(CN)6, 0.13 M EDTA
PH: 6
Temperature: 37
uniprot:Q5FB27 [aldehyde oxidase(Enzyme) wildtype]

O2 + 4_[[(7S)_7_Hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile + H2O -> H2O2 + Oxidized_4_[[(7S)_7_hydroxy_6,7_dihydro_5H_cyclopenta[e]pyrimidin_4_yl]amino]benzonitrile		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M phosphate, 1.0 mM K3Fe(CN)6, 0.13 M EDTA
PH: 6
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY66-201 nicotine degradation IV
BioCyc:MOUSE_PWY66-221 nicotine degradation III
BioCyc:MOUSE_PWY66-201 nicotine degradation II
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-MMU-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-RNO-964975 Vitamin B6 activation to pyridoxal phosphate
WikiPathways:WP5188 Retinol metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
WikiPathways:WP4507 Molybdenum cofactor (Moco) biosynthesis
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0000609 Mercaptopurine Metabolism Pathway
PathBank:SMP0000017 Vitamin B6 Metabolism
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000503 Hypophosphatasia
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000628 Nicotine Metabolism Pathway
PathBank:SMP0087453 Vitamin B6 Metabolism
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0087456 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0000048 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0000424 Citalopram Action Pathway
PathBank:SMP0000431 Nicotine Action Pathway
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000627 Citalopram Metabolism Pathway
PathBank:SMP0087451 Tryptophan Metabolism