EC: 1.2.1.27

methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (2-methyl-3-oxopropanoate:NAD+ 3-oxidoreductase (CoA-propanoylating))

enzyme lambda metabolic reaction experiment
uniprot:Q02253 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

Coenzyme_A + NAD+ + (S)_Methylmalonate_semialdehyde -> NADH + H+ + Propanoyl_CoA + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 30 mM sodium pyrophosphate, 2 mM dithiothreitol
PH: 8
Temperature: 30
uniprot:Q02253 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

(S)_Methylmalonate_semialdehyde + Coenzyme_A + NAD+ -> Propanoyl_CoA + NADH + H+ + CO2		 (( (Vmax * C) ) / (( (Km * (1 + (I / Ki))) ) + C)) buffer: 30 mM sodium pyrophosphate, 2 mM dithiothreitol
PH: 8
Temperature: 30
uniprot:Q02253 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

(S)_Methylmalonate_semialdehyde + Coenzyme_A + NAD+ -> NADH + H+ + CO2 + Propanoyl_CoA		 (( (Vmax * B) ) / (Km + B)) buffer: 30 mM sodium pyrophosphate, 2 mM dithiothreitol
PH: 8
Temperature: 30
uniprot:Q02253 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

Coenzyme_A + (S)_Methylmalonate_semialdehyde + NAD+ -> NADH + Propanoyl_CoA + H+ + CO2		 (( (Vmax * C) ) / (Km + C)) buffer: 30 mM sodium pyrophosphate, 2 mM dithiothreitol
PH: 8
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

NAD+ + Coenzyme_A + 2_Methyl_3_oxopropanoate -> CO2 + H+ + NADH + Propanoyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) mutant C49A/C176A/C305A/C369A/C403A]

Coenzyme_A + 2_Methyl_3_oxopropanoate + NAD+ -> CO2 + Propanoyl_CoA + NADH + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) mutant C49A/C176A/C305A/C369A/C403A]

2_Methyl_3_oxopropanoate + NAD+ + Coenzyme_A -> CO2 + H+ + Propanoyl_CoA + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

NAD+ + Coenzyme_A + 2_Methyl_3_oxopropanoate -> Propanoyl_CoA + CO2 + NADH + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

NAD+ + Coenzyme_A + 2_Methyl_3_oxopropanoate -> H+ + NADH + Propanoyl_CoA + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) mutant C49A/C176A/C305A/C369A/C403A]

NAD+ + 2_Methyl_3_oxopropanoate + Coenzyme_A -> CO2 + NADH + Propanoyl_CoA + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

NAD+ + Coenzyme_A + 3_Oxopropanoate -> Acetyl_CoA + H+ + NADH + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

3_Oxopropanoate + NAD+ + Coenzyme_A -> NADH + H+ + CO2 + Acetyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30
uniprot:P42412 [methylmalonate-semialdehyde dehydrogenase (acylating)(Enzyme) wildtype]

Coenzyme_A + 3_Oxopropanoate + NAD+ -> CO2 + NADH + H+ + Acetyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 8.2
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-7237 myo-, chiro- and scyllo-inositol degradation
BioCyc:META_P562-PWY myo-inositol degradation I
BioCyc:META_VALDEG-PWY L-valine degradation I
BioCyc:HUMAN_VALDEG-PWY valine degradation
BioCyc:MOUSE_PWY3DJ-86 valine degradation
BioCyc:BSUB_PWY-7237 myo-, chiro- and scyllo-inositol degradation
BioCyc:BSUB_P562-PWY myo-inositol degradation I
Reactome:R-CEL-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-70895 Branched-chain amino acid catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-70895 Branched-chain amino acid catabolism
Reactome:R-RNO-70895 Branched-chain amino acid catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-70895 Branched-chain amino acid catabolism
Reactome:R-DME-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4225 Pyrimidine metabolism and related diseases
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120698 GABA-Transaminase Deficiency
PathBank:SMP0120734 Malonic Aciduria
PathBank:SMP0120778 Carnosinuria, Carnosinemia
PathBank:SMP0120786 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000016 Propanoate Metabolism
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000492 Ureidopropionase Deficiency
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0087234 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120777 Ureidopropionase Deficiency
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000201 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0087180 beta-Alanine Metabolism
PathBank:SMP0087341 Propanoate Metabolism
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120748 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000351 GABA-Transaminase Deficiency
PathBank:SMP0000493 Carnosinuria, Carnosinemia
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0002460 Valine Degradation
PathBank:SMP0087248 Propanoate Metabolism
PathBank:SMP0087284 beta-Alanine Metabolism
PathBank:SMP0087432 beta-Alanine Metabolism
PathBank:SMP0087480 Propanoate Metabolism
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000007 beta-Alanine Metabolism
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000198 Malonic Aciduria
PathBank:SMP0000502 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0002444 Threonine Metabolism
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation