EC: 1.17.3.2

xanthine oxidase (xanthine:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q00519 [xanthine oxidase(Enzyme) wildtype XOX(D)]

NAD+ + all_trans_Retinal + H2O -> Retinoate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 32 mM Tetrasodium pyrophosphate, 0.1 mM Pyrazole, 5 mM Glutathione, 1 mM EDTA
PH: 8.2
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype chymotrypsin & dithioerythritol treated]

Xanthine + O2 + H2O -> Urate + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 0.033 mM Methylene blue
PH: 8.1
Temperature: 25
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

O2 + H2O + Xanthine -> Urate + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 0.033 mM Methylene blue
PH: 8.1
Temperature: 25
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

H2O + O2 + 6_Deoxypenciclovir -> Penciclovir + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:P22985 [xanthine oxidase(Enzyme) wildtype]

H2O + 6_Deoxypenciclovir + O2 -> H2O2 + Penciclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

O2 + H2O + 6_Deoxypenciclovir -> H2O2 + Penciclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 67 mM Sorenson's phosphate, 0.1 mM EDTA
PH: 7
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

Xanthine + O2 + H2O -> Urate + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

O2 + Purine_nucleoside + H2O -> 7,9_Dihydropurin_8_one_ribonucleoside + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

2_Hydroxypurine + H2O + O2 -> H2O2 + 2_Hydroxy_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

Purine_2'_deoxyribonucleoside + H2O + O2 -> H2O2 + 7,9_Dihydropurin_8_one_2'_deoxyribonucleoside		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Allopurinol ribonucleoside]

O2 + 6_Methylaminopurine + H2O -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Alloxanthine]

H2O + 6_Methylaminopurine + O2 -> 6_Methylamino_7,9_dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [4-Methylsulfanyl-1H-imidazo[4,5-c]pyridine]

O2 + H2O + 6_Methylaminopurine -> 6_Methylamino_7,9_dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [3-Deazahypoxanthine]

O2 + 6_Methylaminopurine + H2O -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [1-Deazahypoxanthine]

O2 + H2O + 6_Methylaminopurine -> 6_Methylamino_7,9_dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Purine_nucleoside + O2 + H2O -> H2O2 + 7,9_Dihydropurin_8_one_ribonucleoside		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

H2O + O2 + 2_Aminopurine_2'_deoxyribonucleoside -> H2O2 + 2_Amino_7,9_dihydropurin_8_one_2'_deoxyribonucleoside		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

O2 + 2_Aminopurine + H2O -> 2_Amino_7,9_dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

2_Aminopurine_ribonucleoside + O2 + H2O -> 2_Amino_7,9_dihydropurin_8_one_ribonucleoside + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

Hypoxanthine + 2 H2O + 2 O2 -> Urate + 2 H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

2 H2O + 7_Methylhypoxanthine + 2 O2 -> 2 H2O2 + 7_Methylurate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

3_Methylhypoxanthine + 2 O2 + 2 H2O -> 3_Methylurate + 2 H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

2_Aminopurine_2'_deoxyribonucleoside + H2O + O2 -> 2_Amino_7,9_dihydropurin_8_one_2'_deoxyribonucleoside + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

H2O + 6_Methylpurine + O2 -> H2O2 + 6_Methyl_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

2_Aminopurine + H2O + O2 -> H2O2 + 2_Amino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

H2O + O2 + 2_Aminopurine_arabinonucleoside -> H2O2 + 2_Amino_7,9_dihydropurin_8_one_arabinonucleoside		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

O2 + H2O + 2_Aminopurine_ribonucleoside -> 2_Amino_7,9_dihydropurin_8_one_ribonucleoside + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

H2O + O2 + 6'_Deoxyacyclovir -> 6_Deoxy_8_hydroxyacyclovir + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Xanthine + O2 + H2O -> H2O2 + Urate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [Alloxanthine]

O2 + H2O + 6_Methylaminopurine -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [Acyclovir]

O2 + H2O + 6_Methylaminopurine -> 6_Methylamino_7,9_dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

2 O2 + Hypoxanthine + 2 H2O -> Urate + 2 H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

Purine + O2 + H2O -> 7,9_Dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

H2O + 6_Mercaptopurine + O2 -> H2O2 + 6_Sulfanylidene_7,9_dihydro_3H_purin_8_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

O2 + Allopurinol + H2O -> H2O2 + Alloxanthine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

H2O + O2 + 2_Aminopurine_arabinonucleoside -> 2_Amino_7,9_dihydropurin_8_one_arabinonucleoside + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

6'_Deoxyacyclovir + H2O + O2 -> H2O2 + 6_Deoxy_8_hydroxyacyclovir		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [9-Methylxanthine]

H2O + 6_Methylaminopurine + O2 -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

1_Methylnicotinamide + 2 H2O + 2 O2 -> N1_Methyl_2_pyridone_5_carboxamide + 2 H2O2 + N1_Methyl_4_pyridone_5_carboxamide		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

6_Methylaminopurine + O2 + H2O -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

O2 + H2O + Guanosine -> 8_Hydroxyguanosine + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

H2O + O2 + Deoxyguanosine -> H2O2 + 2'_Deoxy_8_hydroxyguanosine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

H2O + Adenine + O2 -> H2O2 + 6_Amino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

Formycin_B + H2O + O2 -> Oxoformycin_B + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

O2 + H2O + Allopurinol -> H2O2 + Alloxanthine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

Inosine + H2O + O2 -> 8_Hydroxyinosine + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Phenazine ethosulfate]

Xanthine + O2 + H2O -> Urate + H2O2		 (( (Vmax * M) ) / (( (Km + M) ) + (( (M ^ 2) ) / Ki))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

H2O + 6_Methylaminopurine + O2 -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + (S * (1 + (I / Kii))))) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Guanine arabinoside]

6_Methylaminopurine + O2 + H2O -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Guanine]

O2 + H2O + 6_Methylaminopurine -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Acyclovir]

6_Methylaminopurine + H2O + O2 -> 6_Methylamino_7,9_dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Ganciclovir]

6_Methylaminopurine + O2 + H2O -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Theophylline]

6_Methylaminopurine + H2O + O2 -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [Azathioprine]

6_Methylaminopurine + O2 + H2O -> H2O2 + 6_Methylamino_7,9_dihydropurin_8_one		 (( (Vmax * S) ) / (( (Km * (1 + (I / Kis))) ) + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P47989 [xanthine oxidase(Enzyme) wildtype]

Benzaldehyde + H2O + O2 -> H2O2 + Benzoate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

H2O + Purine + O2 -> 7,9_Dihydropurin_8_one + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 0.3 mM EDTA , 0.3 mM phenazine ethosulfate, 0.08 mM cytochrome c, 0.5 mg/ml BSA
PH: 6.8
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Nitrite + H+ + NADH -> Nitric_oxide + NAD+ + H2O		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: -
PH: 7
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

H+ + NADH + Nitrite -> NAD+ + Nitric_oxide + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Nitrite + Xanthine -> Nitric_oxide + Urate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Nitrite + Xanthine -> Nitric_oxide + Urate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: -
PH: 7
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Xanthine + Nitrite -> Urate + Nitric_oxide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7.4
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Nitrite + 2,3_Dihydroxybenzaldehyde -> Nitric_oxide + 2,3_Dihydroxybenzoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: -
PH: 7
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Nitrite + 2,3_Dihydroxybenzaldehyde -> Nitric_oxide + 2,3_Dihydroxybenzoate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: -
PH: 7
Temperature: 37
uniprot:P80457 [xanthine oxidase(Enzyme) wildtype]

Xanthine + Nitrite -> Urate + Nitric_oxide		 (Vmax / (( (1 + (Km / A)) ) + (A / Ki))) buffer: -
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-5695 urate biosynthesis/inosine 5'-phosphate degradation
BioCyc:META_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:META_PWY-5695 inosine 5'-phosphate degradation
BioCyc:MOUSE_URSIN-PWY ureide biosynthesis
BioCyc:HUMAN_PWY-6875 retinoate biosynthesis II
BioCyc:MOUSE_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:HUMAN_PWY-6608 guanosine nucleotides degradation
BioCyc:HUMAN_SALVADEHYPOX-PWY adenosine nucleotides degradation
BioCyc:META_PWY-6608 guanosine nucleotides degradation III
BioCyc:MOUSE_PWY-5695 urate biosynthesis/inosine 5'-phosphate degradation
BioCyc:MOUSE_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:HUMAN_PWY-6353 purine nucleotides degradation
BioCyc:META_PWY-6875 retinoate biosynthesis II
BioCyc:META_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:MOUSE_PWY-6608 guanosine nucleotides degradation III
Reactome:R-RNO-9748784 Drug ADME
Reactome:R-HSA-9748787 Azathioprine ADME
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-RNO-9748787 Azathioprine ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-MMU-9748787 Azathioprine ADME
Reactome:R-GGA-1660598 Metabolism
Reactome:R-HSA-8851680 Butyrophilin (BTN) family interactions
Reactome:R-MMU-168256 Immune System
Reactome:R-MMU-1430728 Metabolism
Reactome:R-RNO-1280218 Adaptive Immune System
Reactome:R-GGA-419470 Nucleotide metabolism
Reactome:R-HSA-74259 Purine catabolism
Reactome:R-MMU-8851680 Butyrophilin (BTN) family interactions
Reactome:R-MMU-74259 Purine catabolism
Reactome:R-RNO-74259 Purine catabolism
Reactome:R-RNO-168256 Immune System
Reactome:R-HSA-168256 Immune System
Reactome:R-MMU-1280218 Adaptive Immune System
Reactome:R-RNO-1430728 Metabolism
Reactome:R-GGA-419474 Purine metabolism
Reactome:R-GGA-421178 Urate synthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-8956319 Nucleotide catabolism
Reactome:R-HSA-1280218 Adaptive Immune System
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-8956319 Nucleotide catabolism
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-8956319 Nucleotide catabolism
Reactome:R-RNO-8851680 Butyrophilin (BTN) family interactions
WikiPathways:WP4792 Purine metabolism
WikiPathways:WP5355 Metabolic Epileptic Disorders
WikiPathways:WP4224 Purine metabolism and related disorders
WikiPathways:WP4507 Molybdenum cofactor (Moco) biosynthesis
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000028 Caffeine Metabolism
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000609 Mercaptopurine Metabolism Pathway
PathBank:SMP0063604 Caffeine Metabolism
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0000650 Doxorubicin Metabolism Pathway
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0063777 Nitric Oxide Signaling Pathway