EC: 1.1.1.22

UDP-glucose 6-dehydrogenase (UDP-alpha-D-glucose:NAD+ 6-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P0C0F4 [UDPxylose]

2 NAD+ + UDPglucose + H2O -> H+ + UDPglucuronate + 2 NADH		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Trien-HCl, 2 mM dithiothreitol
PH: 8.7
Temperature: 30
uniprot:P0C0F4 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + 2 NAD+ + UDPglucose -> UDPglucuronate + H+ + 2 NADH		 (( (( (E * kcat) ) * A) ) / (( (Km * (1 + (Q / KiQ))) ) + A)) buffer: 50 mM Trien-HCl, 2 mM dithiothreitol
PH: 8.7
Temperature: 30
uniprot:P0C0F4 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + 2 NAD+ + UDPglucose -> H+ + UDPglucuronate + 2 NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Trien-HCl, 2 mM dithiothreitol
PH: 8.7
Temperature: 30
uniprot:P0C0F4 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + 2 NAD+ + UDPglucose -> H+ + UDPglucuronate + 2 NADH		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Trien-HCl, 2 mM dithiothreitol
PH: 8.7
Temperature: 30
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype synthetic]

2 NAD+ + UDPglucose + H2O -> H+ + UDPglucuronate + 2 NADH		 (( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM sodium glycine
PH: 8.7
Temperature: 25
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype synthetic]

UDPglucose + 2 NAD+ + H2O -> UDPglucuronate + 2 NADH + H+		 (( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM sodium glycine
PH: 8.7
Temperature: 25
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

2 NAD+ + H2O + UDPglucose -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM sodium glycine
PH: 8.7
Temperature: 25
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + UDPglucose + 2 NAD+ -> H+ + UDPglucuronate + 2 NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM sodium glycine
PH: 8.7
Temperature: 25
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) mutant A222Q/S233G]

H2O + 2 NAD+ + UDPglucose -> 2 NADH + H+ + UDPglucuronate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM sodium glycine
PH: 8.7
Temperature: 25
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) mutant A222Q/S233G]

H2O + UDPglucose + 2 NAD+ -> 2 NADH + H+ + UDPglucuronate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM sodium glycine
PH: 8.7
Temperature: 25
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDP_D_glucose + H2O + 2 NAD+ -> UDPglucuronate + 2 NADH + H+		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-Cl, 50 mM KCl, 10 % (v/v) ethanediol
PH: 8.4
Temperature: 25
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + 2 NAD+ + UDP_D_glucose -> UDPglucuronate + H+ + 2 NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-Cl, 50 mM KCl, 10 % (v/v) ethanediol
PH: 8.4
Temperature: 25
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

2 NAD+ + H2O + UDPglucose -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl
PH: 8
Temperature: 20
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + H2O + 2 NAD+ -> H+ + UDPglucuronate + 2 NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl
PH: 8
Temperature: 20
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype (3 isoenzymes EH, EM, EL)]

H2O + 2 NAD+ + UDPglucose -> UDPglucuronate + 2 NADH + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 32 mM Tris-HCl
PH: 8
Temperature: 25
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype (3 isoenzymes EH, EM, EL)]

UDPglucose + H2O + 2 NAD+ -> H+ + UDPglucuronate + 2 NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 32 mM Tris-HCl
PH: 8
Temperature: 25
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype (3 isoenzymes EH, EM, EL)]

2 NAD+ + H2O + UDPglucose -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl
PH: 8
Temperature: 20
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype (3 isoenzymes EH, EM, EL)]

2 NAD+ + H2O + UDPglucose -> UDPglucuronate + H+ + 2 NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 32 mM Tris-HCl
PH: 8
Temperature: 25
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype (3 isoenzymes EH, EM, EL)]

H2O + UDPglucose + 2 NAD+ -> 2 NADH + UDPglucuronate + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl
PH: 8
Temperature: 20
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + 2 NAD+ + H2O -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Glycine
PH: 8.7
Temperature: 30
- [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + H2O + 2 NAD+ -> UDPglucuronate + 2 NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Glycine
PH: 8.7
Temperature: 30
uniprot:Q9LF33 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + H2O + 2 NAD+ -> 2 NADH + UDPglucuronate + H+		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 40 mM Tris-HCl, 0.8 mM EDTA, 16 % (v/v) glycerol, 0.8 mM NaN3
PH: 8.7
Temperature: 22
uniprot:Q9FM01 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + 2 NAD+ + H2O -> H+ + 2 NADH + UDPglucuronate		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 40 mM Tris-HCl, 0.8 mM EDTA, 16 % (v/v) glycerol, 0.8 mM NaN3
PH: 8.7
Temperature: 22
uniprot:Q9LIA8 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + UDPglucose + 2 NAD+ -> H+ + UDPglucuronate + 2 NADH		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 40 mM Tris-HCl, 0.8 mM EDTA, 16 % (v/v) glycerol, 0.8 mM NaN3
PH: 8.7
Temperature: 22
uniprot:Q9FM01 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + 2 NAD+ + H2O -> H+ + 2 NADH + UDPglucuronate		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 40 mM Tris-HCl, 0.8 mM EDTA, 16 % (v/v) glycerol, 0.8 mM NaN3
PH: 8.7
Temperature: 22
uniprot:Q9LF33 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + 2 NAD+ + UDPglucose -> 2 NADH + H+ + UDPglucuronate		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 40 mM Tris-HCl, 0.8 mM EDTA, 16 % (v/v) glycerol, 0.8 mM NaN3
PH: 8.7
Temperature: 22
uniprot:Q9LIA8 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

2 NAD+ + UDPglucose + H2O -> 2 NADH + UDPglucuronate + H+		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 40 mM Tris-HCl, 0.8 mM EDTA, 16 % (v/v) glycerol, 0.8 mM NaN3
PH: 8.7
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

H2O + 2 NAD+ + UDPglucose -> 2 NADH + H+ + UDPglucuronate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl
PH: 7.4
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

2 NAD+ + UDPglucose + H2O -> 2 NADH + UDPglucuronate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl
PH: 7.4
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) mutant K339A]

UDPglucose + 2 NAD+ + H2O -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.4
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + H2O + 2 NAD+ -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M sodium phosphate
PH: 7.4
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + H2O + 2 NAD+ -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.4
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) mutant D280E]

2 NAD+ + UDPglucose + H2O -> H+ + UDPglucuronate + 2 NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.4
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) mutant D280E]

2 NAD+ + UDPglucose + H2O -> UDPglucuronate + H+ + 2 NADH		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M sodium phosphate
PH: 7.4
Temperature: 22
uniprot:O60701 [UDP-glucose 6-dehydrogenase(Enzyme) mutant K339A]

2 NAD+ + UDPglucose + H2O -> H+ + 2 NADH + UDPglucuronate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M sodium phosphate
PH: 7.4
Temperature: 22
uniprot:P76373 [UDP-glucose 6-dehydrogenase(Enzyme) wildtype]

UDPglucose + 2 NAD+ + H2O -> UDPglucuronate + H+ + 2 NADH		 (( (V * B) ) / (Km + B)) buffer: 125 mM potassium bicinate
PH: 8.5
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-4821 UDP-α-D-xylose biosynthesis
BioCyc:META_PWY-7328 superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis
BioCyc:ECOL199310_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECOL199310_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
BioCyc:MOUSE_PWY-4821 UDP-D-xylose and UDP-D-glucuronate biosynthesis
BioCyc:ECOL316407_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECO_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
BioCyc:ARA_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
BioCyc:ECOL199310_PWY-6415 L-ascorbate biosynthesis V
BioCyc:MOUSE_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:HUMAN_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
BioCyc:META_PWY-7820 teichuronic acid biosynthesis (B. subtilis 168)
BioCyc:META_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:META_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
BioCyc:ECO_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECOO157_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECOO157_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
BioCyc:ECOL199310_PWY3DJ-35471 L-ascorbate biosynthesis IV
BioCyc:MOUSE_PWY3DJ-35471 L-ascorbate biosynthesis VI
BioCyc:ECOL316407_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
BioCyc:HUMAN_PWY-4821 UDP-D-xylose and UDP-D-glucuronate biosynthesis
BioCyc:ECOO157_PWY3DJ-35471 L-ascorbate biosynthesis IV
BioCyc:ECOO157_PWY-6415 L-ascorbate biosynthesis V
PathBank:SMP0000914 Amino Sugar and Nucleotide Sugar Metabolism III
PathBank:SMP0000967 Colanic Acid Building Blocks Biosynthesis
PlantCyc:ARA_PWY-7346 UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
PlantCyc:ARA_PWY-5114 UDP-sugars interconversion
PlantCyc:ARA_PWY-4821 UDP-α-D-xylose biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-CEL-211859 Biological oxidations
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-156588 Glucuronidation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-MMU-156588 Glucuronidation
Reactome:R-RNO-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-BTA-211859 Biological oxidations
Reactome:R-BTA-156588 Glucuronidation
Reactome:R-CEL-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-RNO-156588 Glucuronidation
Reactome:R-CEL-156580 Phase II - Conjugation of compounds
Reactome:R-DME-156580 Phase II - Conjugation of compounds
Reactome:R-HSA-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-MMU-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-RNO-1430728 Metabolism
Reactome:R-BTA-156580 Phase II - Conjugation of compounds
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-156588 Glucuronidation
Reactome:R-DME-211859 Biological oxidations
Reactome:R-DME-156588 Glucuronidation
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-156580 Phase II - Conjugation of compounds
WikiPathways:WP5312 Metabolic pathways of fibroblasts
PathBank:SMP0120838 Glycogenosis, Type VI. Hers Disease
PathBank:SMP0000496 Galactosemia III
PathBank:SMP0000552 Glycogen Synthetase Deficiency
PathBank:SMP0000556 Mucopolysaccharidosis VII. Sly Syndrome
PathBank:SMP0087302 Nucleotide Sugars Metabolism
PathBank:SMP0120781 Galactosemia III
PathBank:SMP0120837 Glycogenosis, Type IV. Amylopectinosis, Anderson Disease
PathBank:SMP0000495 Galactosemia II (GALK)
PathBank:SMP0000555 Glycogenosis, Type VI. Hers Disease
PathBank:SMP0087345 Starch and Sucrose Metabolism
PathBank:SMP0120780 Galactosemia II (GALK)
PathBank:SMP0120836 Glycogenosis, Type III. Cori Disease, Debrancher Glycogenosis
PathBank:SMP0120840 Sucrase-Isomaltase Deficiency
PathBank:SMP0000010 Nucleotide Sugars Metabolism
PathBank:SMP0000553 Glycogenosis, Type III. Cori Disease, Debrancher Glycogenosis
PathBank:SMP0000557 Sucrase-Isomaltase Deficiency
PathBank:SMP0120835 Glycogen Synthetase Deficiency
PathBank:SMP0120839 Mucopolysaccharidosis VII. Sly Syndrome
PathBank:SMP0000058 Starch and Sucrose Metabolism
PathBank:SMP0000554 Glycogenosis, Type IV. Amylopectinosis, Anderson Disease
PathBank:SMP0087439 Nucleotide Sugars Metabolism