EC: 1.14.15.16

vitamin D3 24-hydroxylase (calcitriol,adrenodoxin:oxygen oxidoreductase (24-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:O73684 [vitamin D3 24-hydroxylase(Enzyme) wildtype]

Calcidiol + NADPH + H+ + O2 -> Secalciferol + H2O + NADP+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 15 mM Tris-acetate, 8 µM adrenodoxin, 0.4 µM adrenodoxin reductase, 5 mM glucose 6-phosphate, 2 U/ml glucose 6-phosphate dehydrgenase
PH: 7.4
Temperature: 37
uniprot:Q09128 [vitamin D3 24-hydroxylase(Enzyme) wildtype]

NADPH + H+ + Calcitriol + O2 -> H2O + NADP+ + Calcitetrol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 1 mM EDTA, 0.1 units/ml NADPH-adrenodoxin reductase, 4 µM Adrenodoxin
PH: 7.7
Temperature: 37
uniprot:Q09128 [Dilauroylglyceryl-3-phosphorylcholine]

O2 + Calcidiol + H+ + NADPH -> Secalciferol + NADP+ + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 1 mM EDTA, 0.1 units/ml NADPH-adrenodoxin reductase, 4 µM Adrenodoxin
PH: 7.7
Temperature: 37
uniprot:Q09128 [vitamin D3 24-hydroxylase(Enzyme) wildtype]

NADPH + H+ + O2 + Calcidiol -> NADP+ + Secalciferol + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 1 mM EDTA, 0.1 units/ml NADPH-adrenodoxin reductase, 4 µM Adrenodoxin
PH: 7.7
Temperature: 37
uniprot:Q09128 [vitamin D3 24-hydroxylase(Enzyme) wildtype]

H+ + O2 + Calcidiol + NADPH -> Secalciferol + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 1 mM EDTA, 0.1 units/ml NADPH-adrenodoxin reductase, 4 µM Adrenodoxin
PH: 7.7
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-196791 Vitamin D (calciferol) metabolism
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-196791 Vitamin D (calciferol) metabolism
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-RNO-211916 Vitamins
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-HSA-211916 Vitamins
Reactome:R-MMU-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-MMU-211916 Vitamins
Reactome:R-RNO-196791 Vitamin D (calciferol) metabolism
PathBank:SMP0120629 Aromatase Deficiency
PathBank:SMP0120641 Corticosterone Methyl Oxidase I Deficiency (CMO I)
PathBank:SMP0120649 Apparent Mineralocorticoid Excess Syndrome
PathBank:SMP0120451 Adrenal Hyperplasia Type 5 or Congenital Adrenal Hyperplasia Due to 17 alpha-Hydroxylase Deficiency
PathBank:SMP0120640 21-Hydroxylase Deficiency (CYP21)
PathBank:SMP0063672 Steroidogenesis
PathBank:SMP0120437 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0120630 17-alpha-Hydroxylase Deficiency (CYP17)
PathBank:SMP0120642 Corticosterone Methyl Oxidase II Deficiency (CMO II)
PathBank:SMP0120650 3-beta-Hydroxysteroid Dehydrogenase Deficiency
PathBank:SMP0063594 Androgen and Estrogen Metabolism
PathBank:SMP0120450 Adrenal Hyperplasia Type 3 or Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
PathBank:SMP0120470 Congenital Lipoid Adrenal Hyperplasia (CLAH) or Lipoid CAH
PathBank:SMP0120639 11-beta-Hydroxylase Deficiency (CYP11B1)