EC: 1.14.15.15

cholestanetriol 26-monooxygenase (5beta-cholestane-3alpha,7alpha,12alpha-triol,adrenodoxin:oxygen oxidoreductase (26-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:P17178 [cholestanetriol 26-monooxygenase(Enzyme) wildtype]

NADPH + H+ + 3alpha,7alpha,12alpha_Trihydroxy_5beta_cholestane + O2 -> NADP+ + H2O + 3alpha,7alpha,12alpha,26_Tetrahydroxy_5beta_cholestane		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 60 mM potassium phosphate, 10 µM adrenodoxin, 0.2 U/ml bovine adrenodoxin reductase
PH: 7.7
Temperature: 30
uniprot:P17178 [3alpha,7alpha,12alpha-Trihydroxy-5beta-cholestane]

H+ + NADPH + O2 + 3alpha,7alpha_Dihydroxy_5beta_cholestane -> 3alpha,7alpha,26_Trihydroxy_5beta_cholestane + H2O + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 60 mM potassium phosphate, 10 µM adrenodoxin, 0.2 U/ml bovine adrenodoxin reductase
PH: 7.7
Temperature: 30
uniprot:P17178 [cholestanetriol 26-monooxygenase(Enzyme) wildtype]

H+ + O2 + 3alpha,7alpha_Dihydroxy_5beta_cholestane + NADPH -> NADP+ + H2O + 3alpha,7alpha,26_Trihydroxy_5beta_cholestane		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 60 mM potassium phosphate, 10 µM adrenodoxin, 0.2 U/ml bovine adrenodoxin reductase
PH: 7.7
Temperature: 30
uniprot:C4B644 [cholestanetriol 26-monooxygenase(Enzyme) mutant Vdh-K1 (T70R/V156L/E216M/E384R)]

O2 + NADPH + H+ + Vitamin_D3 -> NADP+ + Calcidiol + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM NaCl, 96 µg/ml ferredoxin, 0.1 U/ml ferredoxin-NADP+ reductase, 3 U/ml glucose dehydrogenase, 2 mM NADH, 60 mM D-glucose, 0.05% partially methylated-beta-cyclodextrin
PH: 7.5
Temperature: 30
uniprot:C4B644 [cholestanetriol 26-monooxygenase(Enzyme) wildtype His-tagged]

O2 + Calcidiol + H+ + NADPH -> H2O + NADP+ + Dihydroxyvitamin_D3		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM NaCl, 96 µg/ml ferredoxin, 0.1 U/ml ferredoxin-NADP+ reductase, 3 U/ml glucose dehydrogenase, 2 mM NADH, 60 mM D-glucose
PH: 7.5
Temperature: 30
uniprot:C4B644 [cholestanetriol 26-monooxygenase(Enzyme) wildtype His-tagged]

O2 + Vitamin_D3 + H+ + NADPH -> Calcidiol + H2O + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM NaCl, 96 µg/ml ferredoxin, 0.1 U/ml ferredoxin-NADP+ reductase, 3 U/ml glucose dehydrogenase, 2 mM NADH, 60 mM D-glucose
PH: 7.5
Temperature: 30
uniprot:C4B644 [cholestanetriol 26-monooxygenase(Enzyme) wildtype His-tagged]

NADPH + Vitamin_D3 + H+ + O2 -> NADP+ + Calcidiol + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM NaCl, 96 µg/ml ferredoxin, 0.1 U/ml ferredoxin-NADP+ reductase, 3 U/ml glucose dehydrogenase, 2 mM NADH, 60 mM D-glucose, 0.05% partially methylated-beta-cyclodextrin
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:MOUSE_PWY-6061 bile acid biosynthesis, neutral pathway
Reactome:R-HSA-194068 Bile acid and bile salt metabolism
Reactome:R-HSA-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-HSA-211976 Endogenous sterols
Reactome:R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-MMU-211976 Endogenous sterols
Reactome:R-RNO-192105 Synthesis of bile acids and bile salts
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-HSA-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-194068 Bile acid and bile salt metabolism
Reactome:R-MMU-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-RNO-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-HSA-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-MMU-192105 Synthesis of bile acids and bile salts
Reactome:R-MMU-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-RNO-194068 Bile acid and bile salt metabolism
Reactome:R-RNO-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
Reactome:R-RNO-211976 Endogenous sterols
WikiPathways:WP4545 Oxysterols derived from cholesterol
PathBank:SMP0120468 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0120629 Aromatase Deficiency
PathBank:SMP0000318 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0120463 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120467 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0120495 Zellweger Syndrome
PathBank:SMP0000035 Bile Acid Biosynthesis
PathBank:SMP0000314 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0000315 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120652 27-Hydroxylase Deficiency
PathBank:SMP0120437 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0120477 Familial Hypercholanemia (FHCA)
PathBank:SMP0000317 Familial Hypercholanemia (FHCA)
PathBank:SMP0063594 Androgen and Estrogen Metabolism
PathBank:SMP0063688 Androstenedione Metabolism
PathBank:SMP0000316 Zellweger Syndrome
PathBank:SMP0000720 27-Hydroxylase Deficiency
PathBank:SMP0063601 Bile Acid Biosynthesis